一株1型非洲马瘟疫苗毒种的初步鉴定

Preparation and Primary Identification of One African Horse Sickness Virus Serotype 1 Vaccine Seed

为做好非洲马瘟疫苗的储备性研究,以更好应对我国周边地区非洲马瘟疫情传入风险,对中国兽医微生物菌种保藏管理中心的一株1型非洲马瘟病毒鼠脑组织毒进行了复壮和细胞适应性培养,并对其病毒含量、特异性、对小鼠毒力等进行测定。结果显示,该毒种在Vero细胞连续传代至F10代后病变产生明显,病毒含量可稳定在10^(6.5) TCID_(50)/0.1mL以上;对小鼠毒力表现稳定,细胞适应毒也可达10^(6.5) TCID_(50)/0.1mL。根据OIE参考引物进行RT-PCR鉴定,序列比对证实该毒株为血清1型,与泰国毒株THA2020/01拥有99.7%的同源性。血清学试验结果表明,该毒株可被非洲马瘟1型特异性血清中和。本研究获得了一株Vero细胞适应毒,可作为良好的疫苗毒株候选株,适用于规模化生产,为疫苗储备奠定良好基础。

In order to better tackle with the risk of introduction of African Horse Sickness from neighboring countries and to maintain the vaccine emergency stockpile,one African Horse Sickness Virus(AHSV)serotype 1 vaccine seed was prepared by rejuvenation and adaptation in Vero cells,and its characteristics,viral titers,virulence in mice and specificity were determined.Results showed that this seed strain has a good characteristic.Virus titers could reach 10^(6.5) TCID_(50)/0.1mL,as well as the virulence in mice reaching 10^(6.5) TCID_(50)/0.1mL.Furthermore,sequence and alignment analysis indicated that the strain shares 99.7%homology of nucleotide sequence with isolate THA2020/01,and serum neutralization test showed that it could be neutralized by specific serum,suggesting this prepared culture could be a good candidate as a vaccine strain,laying the foundation for subsequent research.