人类离体疾病肝脏模型的构建

Construction of liver model of human disease in vitro

目的使用常温机械灌注技术,建立人类离体疾病肝脏模型。方法将1例来自肝移植患者肝硬化肝脏的肝动脉和门静脉连接至Life-X100灌注系统进行常温机械灌注。持续监测灌注压力、流量以及灌注液血气分析结果,并分析疾病肝脏功能。灌注前后肝组织做H&E染色。结果成功离体灌注疾病肝脏100 h,灌注参数稳定,灌注液血气维持在生理范围。灌注过程中胆汁持续生成,表示胆道功能良好。结论我们成功初步建立了人类离体肝硬化肝脏模型,这是人类首个针对疾病全肝而建立的模型,其为器官医学的研究搭建了合适的平台。

Objective A human liver model with disease was constructed based on normothermic machine perfusion.Methods A cirrhotic liver from a liver transplant patient was studied.Hepatic artery and portal vein were connected to life-X100 technology for normothermic machine perfusion.We continuously monitored the pressure of the flow and blood gas analysis from the perfusate and analyzed the function of the liver.Liver tissue was stained with H&E before and after perfusion.Results We succeeded in making perfusion for 100 h to preserve diseased liver,with stable perfusion parameters,and the blood gas of perfusate maintained within the physiological range.Continuous bile production during perfusion indicates good biliary function.Conclusion We have preliminarily established a human liver model of liver cirrhosis in vitro,which is the first human model for whole liver disease.It has set up a suitable platform for the research of organ medicine.