黑斑原鮡(Glyptosternum maculatum)皮肤RNA-Seq数据中补体样组分分析

Analysis on Complement-like Components in Glyptosternum Maculatum based on the Transcriptome Datasets

黑斑原鮡为我国雅鲁藏布江特有鱼类,其皮肤补体系统在适应低温、急流水域特性中发挥着重要的作用。文章基于前期RNA-Seq数据检索黑斑原鮡(Glyptosternum maculatum)皮肤补体样组分,并对其结构域及高温应激后的基因表达水平进行了分析。结果表明:共获得79条补体样成分unigene,分别编码1个C1q结构域蛋白、1个含C1r结构域蛋白、3个纤维蛋白原相关蛋白、10个丝氨酸蛋白酶、11个含硫酯键蛋白、7个补体受体、14个补体调节因子及4个纤胶凝蛋白。结构域分析表明:黑斑原鮡补体样组分均含有相应的保守结构域,与其他鱼类补体组分的研究结果基本一致。经15℃养殖水温刺激后,79条补体样组分unigene中共有47条unigene表达上调,其中8条为模式识别受体unigene,结合其他鱼类补体组分的研究结果,推测黑斑原鮡已形成了一个含有多种补体组分的补体系统,且具有凝集素途径或替代途径行使补体的生物学功能。

Glyptosternum maculatum is an endemic fish to the Yalong River in China,and its complement system plays an important role in adapting to the characteristics of cold,jet stream watering area.The complement com⁃ponents of G.maculatum were studied based on the RNA-Seq data,and the conserved domains and expression levels after high temperature challenge were analyzed.Seventy-nine complement-like component unigenes were found in RNA-Seq data,encoding 1C1q domain-containing proteins,1C1r domain-containing proteins,3 fibrin⁃ogen-related proteins,10 serine proteases,11 thioester-containing proteins,7 complement receptors,14 comple⁃ment factor,and 4 ficolins.The complement-like components of G.maculatum contained corresponding con⁃served domains as revealed in domain analysis,which is consistent with the results of complement components from other fishes.The expression levels of 47 unigenes were up-regulated by high temperature challenge,of which 8 were pattern recognition receptors.Combining the results of studies on the freshwater fishes,we infer that a primitive complement system containing multiple components exists in G.maculatum,and performs biolog⁃ical functions by the lectin pathway or alternative pathway.The results provide a new basis for understanding the adapting mechanisms to cold and jet stream water of G.maculatum and other fishes,and for exploring the evolu⁃tion of the complement system.