miR-204靶向SDF1/CXCR4通路调控非小细胞肺癌细胞增殖、凋亡及侵袭

Mechanism of miR-204 targeting SDF1/CXCR4 pathway to regulate the proliferation,apoptosis and invasion of nonsmall cell lung cancer cells

目的 探讨miR-204靶向基质细胞衍生因子(SDF1)/CXCR4通路调控非小细胞肺癌(NSCLC)细胞增殖、凋亡及侵袭的机制。方法 将A549细胞随机分为对照组、miR-204 mimic组、miR-204 inhibitor组,通过转染来抑制或过表达miR-204,分别对各组细胞的生物学行为进行检测,通过双荧光素酶报告来验证miR-204与SDF1/CXCR4的靶向关系。结果 miR-204 mimic组细胞的miR-204水平和凋亡率显著高于对照组,增殖、侵袭、SDF1和CXCR4蛋白水平显著低于对照组(P<0.05);miR-204 inhibitor组细胞的miR-204水平和凋亡率显著低于对照组,增殖、侵袭、SDF1和CXCR4蛋白水平显著高于对照组(P<0.05);双荧光素酶报告实验结果显示miR-204和SDF1/CXCR4之间存在靶向结合。结论 miR-204可能通过靶向SDF1/CXCR4通路抑制NSCLC细胞的增殖和侵袭,并促进凋亡。

Objective To explore the mechanism of microRNA(miR)-204 targeting stromal cell-derived factor 1(SDF1)/CXCR4 pathway to regulate the proliferation,apoptosis and invasion of non-small cell lung cancer cells.Methods A549 cells were divided randomly into 3 groups:control group,miR-204 mimic group,miR-204 inhibitor group. miR-204was inhibited or overexpressed by transfection. The biological behaviors of each group of cells were tested. The target relationships between miR-204 and SDF1/CXCR4 were verified by dual luciferase report.Results The miR-204 level and apoptosis rate of the miR-204 mimic group were significantly higher than those of the control group,and the proliferation,invasion,SDF1 and CXCR4 protein levels were significantly lower than those of the control group(P<0.05). The miR-204 level and apoptosis rate of cells in the miR-204 inhibitor group were significantly lower than those of the control group,and the protein levels of proliferation,invasion,SDF1 and CXCR4 were significantly higher than those of the control group(P<0.05).The results of the dual luciferase report experiment showed that there were targeted bindings between miR-204 and SDF1/CXCR4.Conclusion miR-204 may inhibit the proliferation and invasion of NSCLC cells and promote apoptosis by targeting the SDF1/CXCR4 pathway.