模拟增温对青海湖河源湿地土壤微生物群落特征的影响

Effects of simulated warming on soil microorganisms community characteristics in source wetland of qinghai lake

湿地生态系统在生物多样性保护等方面有着重要的功能及地位,不同的湿地生态系统在功能上存在差异,土壤微生物在湿地生态系统中发挥着重要作用,但目前对河源湿地的土壤微生物群落开展的研究较少。全球变暖大背景下,为探究温度升高对河源湿地土壤微生物的影响,利用高通量测序方法来深入了解模拟增温后土壤细菌及真菌的群落结构及多样性的变化。青海湖河源湿地细菌的优势菌群为变形菌门、酸杆菌门、放线菌门及厚壁菌门,真菌的优势菌群为子囊菌门、担子菌门。细菌群落对比真菌群落而言对土壤增温的响应更为明显,细菌菌群的相对丰度呈增加趋势,真菌群落仅Hypocreales目相对丰度显著增加;土壤细菌及真菌群落的丰富度均降低,而群落多样性增加。增温影响了土壤细菌及真菌的群落结构及多样性,且细菌群落对土壤增温更为敏感。

Wetland ecosystems play an important role in biodiversity conservation,but different wetland ecosystems have different functions,and soil microbes play an important role in wetland ecosystems.However,few studies have been carried out on soil microbial communities in source wetland.In the context of global warming,in order to explore the impact of temperature rise on soil microorganisms in source wetland,high-throughput sequencing method was used in this experiment to further understand the community structure and diversity changes of soil bacteria and fungi after simulated warming.The dominant flora were Proteobacteria,Acidobacteria,Actinomycetes and Firmicutes,and the dominant flora were Ascomycetes and Basidiomycetes.Compared with the fungal community,the bacterial community showed a more obvious response to the increase in soil temperature.The relative abundance of the bacterial community was increasing,and the relative abundance of the fungal community was only significantly increased in Hypocreales.The richness of soil bacteria and fungi community decreased,while the community diversity increased.Temperature increase affects the community structure and diversity of soil bacteria and fungi,and the bacterial community is more sensitive to soil temperature increase.