短花针茅StbCO和StbFT基因全长的克隆与表达差异分析

Cloning and Differential Expression Analysis of Full-lengthStbCO and StbFT Genes in Stipa breviflora

光周期调控在植物从营养生长到生殖生长的转变过程中起重要作用,CO和FT基因位于光周期途径的关键位点。参照转录组数据对响应短花针茅开花的主要调控基因StbCO和StbFT基因进行了克隆、亚细胞定位和表达分析,研究短花针茅植物开花与气候变化的响应规律。结果表明,StbCO和StbFT基因全长分别为2065bp和1389bp,分别包含1179bp和534bp的完整编码区。亚细胞定位发现,StbCO蛋白位于细胞核,StbFT蛋白分布于细胞质和细胞核。增温和施氮处理下,StbCO和StbFT基因在短花针茅开花的不同时期表达量有较大差异,为进一步解析其在短花针茅开花过程中的功能提供了有用的信息。

Photoperiod regulation plays an important in the transformation of plants from vegetative growth to reproductive growth.CO and FT genes are key regulatory sites in the photoperiod pathway.In this paper,we cloned,subcellular located and expressed StbCO and StbFT genes,the main regulatory genes of Stipa breviflora in response to flowering,with reference to transcriptome data,in order to study the response of Stipa breviflora to flowering and climate change.The results showed that the full length of StbCO and StbFT genes were 2065bp and 1389bp,respectively,including 1179bp and 534bp complete coding regions.Analysis of subcellular localization revealed that StbCO protein was located in the nucleus,while StbFT protein was distributed in the cytoplasm and nucleus.The expression levels of StbCO and StbFT genes were significantly different at different flowering stages of Stipa breviflora under warming and nitrogen application treatments,which provided useful information for further analysis of their functions in the flowering process of Stipa breviflora.