带血管骨髓移植加速部分清髓受体的造血系统重建

Vascularized bone marrow transplantation accelerates hematopoietic reconstruction of nonmyeloablative receptors

目的利用带血管骨髓移植方法,同时移植骨髓细胞和其天然的微环境以加速重建部分清髓大鼠的造血系统。方法以前期构建的大鼠股骨移植模型作为骨髓的载体,将其移植给经5 Gy放射清髓的同品系Lewis大鼠,通过血常规、脾指数测定、骨髓细胞计数、流式和组织病理等方法评估受体的造血恢复情况,并分别以静脉注射等量骨髓细胞或生理盐水作为阳性对照和阴性对照组。结果与阴性对照组相比,带血管的骨髓和静注的骨髓均显著加速了部分清髓大鼠白细胞和血小板的恢复;脾脏指数高于对照组(P<0.05),HE染色虽未见结构清晰的白髓,但可见大量淋巴小结和淋巴细胞;同样,骨髓HE染色也可见稀疏的骨髓细胞,而对照组骨髓基本被空泡性组织所取代;股骨和胫骨内骨髓细胞数也较对照组高一个数量级(P<0.05)。但以上各项指标带血管的骨髓移植与静脉骨髓移植相比差异均无统计学意义。结论带血管的骨髓移植加速部分清髓受体造血系统的重建,可以作为一种新的骨髓移植方式。

Objective To investigate hematopoietic reconstruction of nonmyeloablative receptors after a new strategy of bone marrow transplantation-vascularized bone marrow transplantation(VBMT)containing both bone marrow cells and its own stromal microenvironment.Methods Vascularized femur as a carrier of BMCs was transplanted into irradiated(5 Gy)syngeneic Lewis rats.Routine blood tests,spleen index measurement,bone marrow count,flow cytometry and histopathological examination were used to assess hematopoietic potentialof the receptor.The same amount of BMCs suspensionor saline was injected intravenouslyto use as a positive or negative control group.Results Ascompared with the negative control group,both VBMT and intravenous BMT accelerated the recovery of leukocytes and platelets and had a higher spleen index(P<0.05).Numerous lymphocytes and lymphatic nodule were seen in the spleen section in spite of obscure boundary betweenthe white and the red pulp.Histological appearances of bone marrow also showed scattered BMCs,but the bone marrow was almostreplaced by vacuolar tissuesin the control group.The number of BMCs was a magnitude lower in the control group than in the VBMT and intravenous BMT groups.However there were no significant differences between VBMT and intravenous BMT in the above results.Conclusions Vascularized bone marrow transplantation accelerates reconstruction of partialhematopoietic systeminnonmyeloablative receptors andcan be used as a new transplantation of bone marrow.