精氨酸加压素1b受体敲除对雌性大鼠焦虑和社交行为的影响

Effects of arginine vasopressin 1b receptor knockout on anxiety behavior and social behavior in female rats

目的探究精氨酸加压素1b受体(arginine vasopressin 1b receptor,AvpR1b)基因敲除对雌性大鼠血浆皮质酮含量、焦虑和社交行为的影响。方法以精氨酸加压素1b受体敲除(AvpR1b^(KO))和野生型(wild type,WT)的SD雌性大鼠为研究对象,首先采用原位杂交技术检测WT和AvpR1b^(KO)大鼠大脑室旁核(paraventricular nucleus,PVN)与视上核(supraoptic nucleus,SON)中精氨酸加压素1b受体的表达水平;采用高效液相色谱串联高分辨质谱分析WT和AvpR1b^(KO)大鼠静息状态下血浆皮质酮含量;通过旷场实验中WT和AvpR1b^(KO)大鼠进入中心区域的时间、次数,以及高架十字迷宫实验中WT和AvpR1b^(KO)大鼠进入开放臂的时间和次数来评价大鼠的焦虑水平;通过三箱实验中WT和AvpR1b^(KO)大鼠探索陌生鼠的时间来评价大鼠社交能力;并根据WT和AvpR1b^(KO)大鼠探索熟悉程度不同陌生大鼠的时间来评价大鼠社交新颖性。结果在AvpR1b^(KO)大鼠室旁核与视上核脑区精氨酸加压素1b受体均无表达;静息状态下,AvpR1b^(KO)大鼠血浆中皮质酮含量较WT大鼠无差异(P>0.05);旷场实验中AvpR1b^(KO)大鼠进入中心区域的时间、次数与WT大鼠无差异(P>0.05);高架实验中AvpR1b^(KO)大鼠进入开放臂区域的时间、次数与WT大鼠无差异(P>0.05);在三箱实验中AvpR1b^(KO)大鼠的社交能力指数与WT大鼠的社交能力指数无显著差异(P>0.05);同时AvpR1b^(KO)大鼠的社会新颖指数与WT大鼠的社会新颖指数无显著差异(P>0.05)。结论精氨酸加压素1b受体敲除不影响雌性大鼠静息状态下血浆中皮质酮含量、焦虑水平、社交能力以及社交新颖性。

Objective To investigate the effects of arginine vasopressin 1b receptor(Avpr1b)gene knockout on the plasma corticosterone level,anxiety,and social behaviors in female rats.Methods Female AvpR1b knockout(AvpR1b^(KO))and wildtype(WT)SD rats were used.First,the expression levels of Avpr1b in the paraventricular nucleus(PVN)and supraoptic nucleus(SON)of the brains of WT and AvpR1b^(KO) rats were examined by performing in situ hybridization.The basal plasma corticosterone levels of WT and AvpR1b^(KO) rats were then analyzed by conducting highperformance liquid chromatography tandem high-resolution mass spectrometry.Additionally,the anxiety levels of rats were evaluated by using an open field test and elevated plus maze test.Finally,the social ability and social novelty of WT and AvpR1b^(KO) rats were measured by performing a three-chamber experiment.Results There was no expression of AvpR1b in paraventricular nucleus or supraoptic nucleus of AvpR1b^(KO) rats.The basal plasma corticosterone level of AvpR1b^(KO) rats was not significantly different from that of WT rats(P>0.05).The duration and bouts of AvpR1b^(KO) rats entering the central area in the open field experiment were not different from those of WT rats(P>0.05).Similarly,the duration and bouts of AvpR1b^(KO) rats entering the open arm area in the elevated plus maze were not different from those of WT rats(P>0.05).In the social interaction experiment,the socialbility index of AvpR1b^(KO) rats was not different from that of WT rats(P>0.05).In the social novelty experiment,the social novelty index of AvpR1b^(KO) rats was not different from that of WT rats(P>0.05).Conclusions A knockout of AvpR1b in female SD rats does not affect their basal plasma corticosterone level,anxiety level,sociability,or interest in social novelty.