KIAA1456靶向调控Runx1抑制肺癌A549细胞增殖和侵袭

KIAA1456 inhibit proliferation and invasion of non-small cell lung cancer A549 cells by targeting Runx1

目的 探讨KIAA1456靶向调控Runx1对非小细胞肺癌A549细胞增殖和侵袭的影响。方法 采用免疫组织化学法检测91例肺癌及癌旁组织中KIAA1456表达情况,分析KIAA1456与肺癌患者临床病理特征的相关性,并进行Kaplan-Meier生存分析。用Lipofectamine法将miR-NC KIAA1456 RNAi和pCDNA3.0-HA-KIAA1456质粒转染A549细胞,MTT法和Transwell法检测细胞增殖和侵袭能力,Western blotting法检测细胞KIAA1456、Cyclin D1、Runx1和MMP-9表达。结果 KIAA1456在肺癌组织中的高表达率明显低于癌旁组织(P<0.05);KIAA1456在不同淋巴结转移、TNM分期分层间表达有差异(P<0.05)。KIAA1456高表达肺癌患者5年总生存率高于KIAA1456低表达者(P<0.05)。与阴性对照组比较,KIAA1456 RNAi组A549细胞增殖率、侵袭细胞数、Cyclin D1、Runx1和MMP-9表达均增加,KIAA1456表达降低;KIAA1456质粒组A549细胞增殖率、侵袭细胞数、Cyclin D1和MMP-9表达均降低,KIAA1456和Runx1表达增加(P<0.05)。结论 KIAA1456可作为肺癌的抑癌基因,通过靶向调控Runx1表达,抑制A549细胞增殖和侵袭。

Aim To investigate the effect of KIAA1456 targeting Runx1 on the proliferation and invasion of non-small cell lung cancer A549 cells.Methods:Immunohistochemistry was used to detect the expression of KIAA1456 in 91 cases of lung cancer and adjacent tissues,and the correlation between KIAA1456 and the clinicopathological characteris-tics of lung cancer patients was analyzed,and Kaplan-Meier survival analysis was performed.The miR-NC KIAA1456 RNAi and pCDNA30-HA-KIAA1456 plasmid was transfected into A549 cells by Lipofectamine.The cell proliferation rate and invasion ability were detected by MTT and Transwell.Western blotting was used to detect the expression of KI-AA1456,Cyclin D1,Runx1 and MMP-9 in the cells.Results:The expression of KIAA1456 in lung cancer tissues was significantly lower than that in the adjacent tissues(P<0.05).The expression level of KIAA1456 was correlated with lymph node metastasis and TNM stage(P<0.05).The 5-year overall survival rate of lung cancer patients with high expres-sion of KIAA1456 was significantly higher than that of patients with with low expression of KIAA1456(P<0.05).Compared with the negative control group,the proliferation rate,number of invaded cells,Cyclin D1,Runx1 and MMP-9 ex-pression of A549 cells in the KIAA1456 RNAi group were increased,while KIAA1456 expression was decreased,and A549 cell proliferation rate,number of invasion cells,Cyclin D1 and MMP-9 expression in KIAA1456 plasmid group were de-creased,KIAA1456 and Runx1 expression were increased(P<0.05).Conclusion KIAA1456 can act as a tumor sup-pressor gene in lung cancer.It can inhibit the proliferation and invasion of A549 cells by targeting the expression of Runx1.