不同检测方法在结核性脑膜炎诊断中的应用价值分析

Application Value Analysis of Different Detection Methods in Diagnosis of Tuberculous Meningitis

目的 对不同的检测技术,在诊断结核性脑膜炎中的意义进行分析。方法 选取2020年1月-2021年12月贵州省贵阳市公共卫生救治中心收治的276例结核性脑膜炎患者,均实施细菌学检测技术(脑脊液抗酸杆菌涂片、脑脊液分枝杆菌慢速培养)、分子生物学检测方法[脑脊液结核/非结核分枝杆菌核酸检测(PCR检测法)、脑脊液结核分枝杆菌检测及利福平耐药基因检测(Gene Xpert检测)]、免疫学检测方法[PPD试验(结核菌素试验)、外周血T-SPOT·TB(外周血T细胞斑点试验)]、脑脊液常规、生化实验室检测结果等检测,对其检测的结果进行分析。结果 多种方法联合检测,相较于单一方法检测,其对结核性脑膜炎的检出率更高,P<0.05。结论 不同方法检测在结核性脑膜炎诊断应用的价值存在一定的差异性,多种方法相结合,可促进疾病检出率的提高。

Objective To analyze significance of different detection techniques in diagnosis of tuberculous meningitis.Methods The paper chose 276 patients with tuberculous meningitis from January 2020 to December 2021 as research subjects,tested by bacteriological techniques (acid fast bacillus smear in cerebrospinal fluid,slow culture of Mycobacterium in cerebrospinal fluid),molecular biological detection methods[(cerebrospinal fluid tuberculosis/non tuberculosis mycobacterium nucleic acid detection (PCR detection),cerebrospinal fluid Mycobacterium tuberculosis detection and rifampicin resistance gene detection (Gene Xpert detection)),immunological detection methods[PPD test (tuberculin test),peripheral blood T-SPOT TB (peripheral blood T cell spot test)],cerebrospinal fluid routine and biochemical laboratory detection results,etc.Test results were analyzed.Results Detection rate of tuberculous meningitis by multiple methods was higher than that by single method (P<0.05).Conclusion There was difference in value of different methods in diagnosis of tuberculous meningitis,combination of multiple methods can improve detection rate of the disease.