解旋酶依赖性扩增技术研究进展

Research Progress of Helicase Dependent Amplification Technology

解旋酶依赖性扩增技术(helicase-dependent amplification,HDA)作为一种灵敏度高、特异性强、快速高效、便捷精准的核酸等温扩增技术,在病原体检测研究中得到广泛应用,有望应用于国境口岸现场病原体快速检测。本文着重介绍HDA技术的原理、方法和优势,并探讨其在国境口岸重点关注病原体检测中的研究进展。

As a nucleic acid isothermal amplification with high sensitivity,strong specificity,fast,efficient,convenient and accurate,helicase-dependent amplification(HDA)is widely used in pathogen detection and research,and expected to be used for rapid detection of pathogens at the national frontier ports.This paper introduces the principles,methods and advantages of HDA,and discusses the research progress of HDA in the detection of several pathogens,focused on ports.