不同居群黑骨藤种子特性研究

Study on Seed Characteristics of Periploca forrestii Schltr from Different Populations

为了研究黑骨藤种子特性的差异,以不同居群的黑骨藤种子为材料,研究不同居群黑骨藤种子的形态学(百粒重、种子形态和大小)差异、种子活力和萌发特性差异。结果表明,不同居群黑骨藤种子形态无明显差异;种子大小以开阳(H 1)的最大,贵州中医药大学资源圃(H 4)的最小;种子的百粒重以开阳的最重最饱满;种子活力测定的最佳条件为:TTC浓度1.0%,染色8 h,染色温度25℃。相关性分析表明,发芽势、发芽指数与萌发率均呈极显著正相关(p<0.01);萌发率与萌发时滞呈显著正相关(p<0.05),而与发霉率、吸水率呈显著负相关(p<0.05)。研究表明,务川县(H 3)和西秀区(H 9)两个居群的种子可作为黑骨藤野生优质种源进行开发利用。

To study the difference in seed characteristics of Periploca forrestii from different populations,the difference in seeds morphology(100-grain weight,seed shape and size),seed vigor and germination characteristics of the seeds from different populations were determined in this study.The results showed that there was no significant difference in seed morphology among different populations;the seeds from Kaiyang(H 1)had the largest size,and the seeds from nursery of Guizhou University of Chinese Medicine(H 4)had the smallest size;the seeds of 100-grain weight from Kaiyang were heaviest and fullest.The optimal conditions for seed vigor were as follows:TTC concentration 1.0%,dyeing for 8 h,dyeing temperature 25℃.Further,the correlation analysis showed that germination potential and germination index were all extremely significantly positively correlated with germination rate(p<0.01);and germination time delay was significantly positively correlated with germination rate(p<0.05),while germination rate was significantly negatively correlated with mildew rate and water absorption rate(p<0.05).Based on the analysis of various indicators,the seeds from the two populations of Wuchuan County(H 3)and Xixiu District(H 9)could be developed and utilized as the wild high-quality germplasms of P.forrestii.