紫外指纹图谱结合化学计量学对黄精基原植物的鉴别

Identification and Analysis of Original Plants of Polygonati Rhizoma by UV Fingerprint Combined with Chemometrics

运用紫外光谱技术结合化学计量学,建立快速鉴别不同基原黄精的方法。通过单因素实验确定黄精最佳提取溶剂、时间和用量,制备测试液,采用紫外光谱技术建立3种基原黄精的紫外指纹图谱,光谱数据转化后进行主成分(PCA)和系统聚类分析(HCA)。该方法重现性、精密度、稳定性较好,结果表明不同种类黄精紫外指纹图谱具有指纹特性,3种基原植物黄精紫外光谱图在210 nm、220 nm、280 nm附近差异明显;聚类分析和主成分分析三维投影图反映出不同种类黄精的化学成分积累具有差异,能较好地区分滇黄精(Polygonatumkingianum)、黄精(P.sibiricum)与多花黄精(P.cyrtonema)。紫外光谱结合化学计量学能快速鉴别不同种类黄精,可作为黄精的鉴别和质量控制新方法,为黄精临床应用、资源开发及黄精属植物分类提供辅助方法。

A method for rapid identification of different original plants of polygonati rhizoma was established by UV spectroscopy combined with chemometrics.The best extraction solvent,time and dosage of polygonati rhizoma were determined by single factor experiment,and the test solution was prepared.The UV fingerprint of three species of Polygonatum was established by UV spectroscopy.After the spectral data were transformed,principal component analysis(PCA)and hierarchical cluster analysis(HCA)were carried out.The method had good reproducibility,precision and stability.The UV fingerprints of three species of original plants of polygonati rhizoma had fingerprint characteristics.Their UV spectra were significantly different at 210 nm,220 nm and 280 nm,and the three-dimensional projection of cluster analysis and principal component analysis showed that the accumulation of chemical components in different species was different.The differences among three species could be distinguished by HCA and PCA.Three species of polygonati rhizoma original plants,i.e.Polygonatum kingianum,P.sibiricum and P.cyrtonema,could be identified rapidly by UV fingerprint with chemometrics.That can provide an auxiliary method for clinical application,resource development and classification of Polygonatum.