摘要
利用PC12细胞建立氧糖剥夺(OGD)体外脑卒中损伤模型,采用该模型对射干中鸢尾甲苷B抗脑卒中进行活性评价。评价指标包括:细胞形态学观察、细胞存活率检测、细胞内活性氧(ROS)含量检测、乳酸脱氢酶(LDH)释放率测定、细胞内钙离子(Ca^(2+))浓度检测、细胞凋亡情况检测等。结果显示,射干中天然异黄酮鸢尾甲苷B能显著减轻氧糖剥夺/复氧(OGD/R)造成的PC12细胞损伤,降低细胞凋亡,提升细胞存活率,降低Ca^(2+)、LDH、ROS水平。结果表明,鸢尾甲苷B对Na_(2)S_(2)O_(4)损伤的PC12细胞有明显的保护作用,其机理可能与脑内神经元的保护作用相关。
PC12 cells were used to establish an in vitro stroke injury model of glucose oxygen deprivation(OGD),and the activity of Iristectorin B in the ejaculatory trunk was evaluated against stroke.The evaluation indexes included the following:cell morphology observation,cell survival rate detection,intracellular reactive oxygen species(ROS)content detection,lactate dehydrogenase(LDH)release rate measurement,intracellular calcium ion(Ca^(2+)) concentration detection,apoptosis situation detection and so on.The results showed that Iristectorin B,a natural isoflavone present in the shoot trunk,significantly attenuated PC12 cell injury caused by oxygen glucose deprivation/reoxygenation(OGD/R),decreased cell apoptosis,elevated cell survival,and decreased Ca^(2+),LDH,and ROS levels.The results showed that Iristectorin B had obvious protective effect on Na_(2)S_(2)O_(4) injured PC12 cells,and the mechanism may be associated with the protective effect of neurons in the brain.
作者
周密
陆瑶
刘惠霞
陈明惠
郑梅竹
ZHOU Mi;LU Yao;LIU Hui-xia;CHEN Ming-hui;ZHENG Mei-zhu(College of Life Sciences,Changchun Normal University,Changchun 130032,China;Central Laboratory,Changchun Normal University,Changchun 130032,China)
出处
《长春师范大学学报》
2022年第6期84-89,共6页
Journal of Changchun Normal University
基金
国家自然科学基金资助项目“药用植物中异黄酮类成分抗缺血性脑卒中的活性筛选及作用机制研究”(31670358)。
