转化有机废弃物的亮斑扁角水虻虫粉饲喂斑马鱼和大鼠的安全性研究

Safety of insect powder feeding zebrafish and rats from Hermertia illucens L.transformed with organic wastes

为对亮斑扁角水虻转化不同物料获得的虫粉的安全性作出评估,探究饲喂亮斑扁角水虻转化不同有机废弃物获得的虫粉后斑马鱼和大鼠生理、生化和组织病理指标的差异。将斑马鱼和大鼠均分为4组:①对照组(斑马鱼对照组,CK1:投喂绿藻粉;大鼠对照组,CK2:投喂常规鼠饲料);②鸡粪饲养组(鸡粪组,A组):投喂鸡粪饲养的亮斑扁角水虻虫粉;③餐厨垃圾饲养组(餐余组,B组):投喂餐厨垃圾饲养的亮斑扁角水虻虫粉;④农副产品饲料饲养组(饲料组,C组):投喂农副产品饲料饲养的亮斑扁角水虻虫粉。斑马鱼孵化后喂养15 d、大鼠喂养28 d后测生理、生化和组织病理指标。结果显示,除鸡粪组外其他各组对斑马鱼生理、生化指标并无显著影响;对照组、餐余组和饲料组水虻虫粉对斑马鱼和大鼠组织病理无显著影响;各组水虻虫粉对大鼠的生理生化指标虽有个别显著影响但均维持在正常值。结果表明,完全以亮斑扁角水虻转化餐厨垃圾和农副产品饲料获得的虫粉可作为高营养的斑马鱼开口饲料和大鼠短期饲养的饲料。

The safety of feeding zebrafish and rats with insect powder obtained from different materials transformed by Hermertia illucens L.was evaluated to compare the differences in physiological,biochemical and histopathological indicators of zebrafish and rats.Zebrafish and rats were divided into four groups including the control group made up of zebra fish fed with algae powder(CK1)and rats fed with traditional rat diet(CK2),chicken excrement feeding group fed with Hermertia illucens L.powder reared with chicken excrement(A group),kitchen waste feeding group fed with Hermertia illucens L.powder reared with kitchen waste(B group),and agricultural by-product feeding group fed with Hermertia illucens L.reared with agricultural by-product(C group).The physiological,biochemical and histopathological indicators of zebrafish and rats were measured on 15 days and 28 days after feeding,respectively.The results showed that except for the A group,other groups had no significant effect on the physiological and biochemical indicators of zebrafish.The control,B and C group had no significant effect on the histopathology of zebrafish and rats.The insect powder in each group had significant effects on the physiological and biochemical indicators of rats,but they were all maintained at normal values.It is indicated that insect powder obtained entirely from the transformation of the kitchen waste and agricultural by-products by Hermertia illucens L.can serve as the open feed with high-nutrient for zebrafish and feed for short-term feeding of rats.