基于UPLC法快速测定前胡配方颗粒中指标性成分

Simultaneous Determination of Effective Components in Decursivum Dispensing Granules by UPLC

目的建立超高效液相法(UPLC)对前胡配方颗粒中白花前胡甲素、白花前胡乙素和白花前胡素E同时快速定量分析的方法。方法采用Acquity UPLC BEH C 18色谱柱(50 mm×2.1 mm,1.7μm),以甲醇-水为流动相,梯度洗脱,体积流量0.4 mL·min^(-1),在321 nm波长处进行检测,柱温25℃,进样量2.0μL。结果前胡配方颗粒中3种有效成分在考察线性范围内线性关系良好,白花前胡甲素、白花前胡乙素、白花前胡素E的线性范围分别为1.870~18.70μg·mL^(-1)(r=0.9998)、0.2075~2.075μg·mL^(-1)(r=0.9998)、0.2220~2.220μg·mL^(-1)(r=0.9997),加样回收率均在98.85%~99.76%,RSD均小于2.0%。结论该方法简便,准确,快速,重复性好,可用于前胡配方颗粒的快速质量控制,并为将来该配方颗粒的质量标准提升提供参考。

OBJECTIVE To develop a UPLC method for fast and simultaneous determination of praeruptorin A,praeruptorin B and qianhucoumarin E in Decursivum Dispensing Granules.METHODS An Acquiyt UPLC BEH C 18 column(50 mm×2.1 mm,1.7μm)was used for the separation,and the mobile phase was in the program of gradient elution consisting of methanol and water with the flow rate of 0.4 mL·min^(-1).The detection wavelength was 321 nm with the column temperature of 25℃.RESULTS The calibration curves of praeruptorin A,praeruptorin B and qianhucoumarin E were linear within the ranges of 1.870-18.70μg·mL^(-1)(r=0.9998),0.2075-2.075μg·mL^(-1)(r=0.9998),0.2220-2.220μg·mL^(-1)(r=0.9997),respectively.The average recovery rates were 99.76%,98.96%and98.85%with the RSD of 1.5%,1.7%and 1.8%(n=6),respectively.CONCLUSION The method is simple,rapid,accurate,good repeatability,and can be used for the quality control of Decursivum Dispensing Granules,and also provide reference for the quality improvement of the Dispensing Granules.