蜂斗菜总内酯对常压缺氧小鼠的保护作用研究

Protective effects of the total bakkenolides from Petasites tricholobus on hypoxia mice under normobaric pressure

目的探讨蜂斗菜总内酯(PTB)对提高动物耐缺氧能力的作用。方法建立小鼠常压缺氧模型和PC12细胞氧糖剥夺模型(OGD),观察PTB对小鼠常压密闭空间下存活时间、血清乳酸脱氢酶(LDH)活性和丙二醛(MDA)含量,对脑组织与心脏超氧化物歧化酶(SOD)和还原型谷胱甘肽(GSH)的活性,以及对脑组织病理变化和细胞存活率的影响。结果PTB可以显著提高常压缺氧小鼠在密闭空间下的存活时间,提高机体SOD、GSH的活性,减少脂质过氧化物的产生,降低无氧酵解的程度,保护神经细胞的结构和功能,提高OGD处理后的细胞的存活率。结论PTB具有明显的提高小鼠耐缺氧能力的作用,其作用机制可能与清除氧自由基、抑制脂质过氧化反应、保护神经细胞结构和功能有关。

Objective To investigate the protective effects of the total bakkenolides from P.tricholobus on improving hypoxia tolerance in mice.Methods Mice normobaric pressure hypoxia model and oxygen glucose deprivation model in PC12 cells were established,and the effects of PTB on survival time,serum lactate dehydrogenase(LDH)activity and malondialdehyde(MDA)content,brain and heart superoxide dismutase(SOD)and reduced glutathione(GSH)activities,brain tissue pathological changes and cell survival were observed.Results The total bakkenolides from P.tricholobus had prolonged the survival time of mice in confined spaces,increased the activity of SOD and GSH,reduced the production of lipid peroxidation,decreased the degree of anaerobic glycolysis,protected the structure and function of neural cells,and improved the survival rate of OGD-treated cells.Conclusion The total bakkenolides from P.tricholobus could promote the hypoxia tolerance in mice which might be related to scavenging oxygen free radicals,inhibiting lipid peroxidation reaction and protecting the structures and functions of nerve cells.