摘要
利用生物信息技术鉴定了桃中漆酶(LAC)家族成员,分析其进化关系分析、基因结构、启动子区顺式作用元件以及表达模式。结果表明,在桃基因组中共鉴定出48个漆酶基因,通过在桃子叶愈伤组织中的表达模式分析,发现1个表达量很高的关键成员PpLAC21,在该基因沉默的桃愈伤组织中,木质素的含量下降,推测该基因可能参与桃愈伤组织的木质素合成。
The members of laccase(LAC)family in peach[Prunus persica L.(Batsch)]were identified by bioinformatics technology,and their evolutionary relationship,gene structure,cis-elements in promoter region and expression pattern were analyzed.The results showed that a total of 48 laccase genes were identified in peach genome.Through the analysis of expression pattern in peach cotyledon callus,it was found that a key member with high expression,PpLAC21,decreased the content of lignin in peach callus silenced by this gene.It is speculated that this gene may be involved in lignin synthesis in peach callus.
作者
邱可立
王玉民
何金铃
俞红
潘海发
盛玉
谢庆梅
陈红莉
周晖
张金云
QIU Keli;WANG Yumin;HE Jinling;YU Hong;PAN Haifa;SHENG Yu;XIE Qingmei;CHEN Hongli;ZHOU Hui;ZHANG Jinyun(Key Laboratory of Anhui Genetic Improvement and Ecophysiology of Horticultural Crop,Instisute of Horticulture,Anhui Academy of Agricultural Sciences,Hefei 230031,China;School of Life Science,Anhui Agricultural University,Hefei 230036,China;School of Biological and Food Engineering,Bozhou University,Bozhou,Anhui 236800,China;School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei 230036,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2022年第6期1351-1362,共12页
Acta Horticulturae Sinica
基金
国家现代农业产业技术体系建设专项资金项目(CARS-30-Z-05)
安徽省果树产业技术体系项目(AHNYCYTX-10)
安徽省重点研究和开发计划项目(202104a06020023,201904a06020047)。
