中等静磁场对CD8^(+)T细胞氨基酸代谢的影响和作用机制

Effects and mechanism of moderate static magnetic field on the amino acid metabolism of CD8^(+)T cells

为探讨中等静磁场对CD8^(+)T细胞氨基酸代谢的影响和作用机制,采用质谱仪检测了0.3 T永久磁铁处理对体外刺激的CD8^(+)T细胞氨基酸浓度的影响。结果表明,在42种氨基酸中有4种氨基酸的浓度在磁场处理的CD8^(+)T细胞中显著上调,即牛磺酸(Tau)、谷氨酸(Glu)、1-甲基-L-组氨酸(1MHis)和精氨琥珀酸(Asa)。随后,在培养基中添加氨基酸体外刺激培养结果发现,在添加1μmol/L牛磺酸或者谷氨酸的条件下能够显著增强CD8^(+)T细胞颗粒酶Granzyme B的分泌。采用荧光定量PCR检测磁场对代谢相关基因mRNA表达水平的影响,以及流式细胞术分析营养受体分子细胞表面的表达情况。结果表明,磁场上调了氨基酸的转运受体CD98和铁的转运受体CD71的表达。以上结果说明,磁场有可能通过上调营养受体的表达来影响CD8^(+)T细胞的氨基酸代谢。

To explore the effects and mechanisms of moderate static magnetic field on the amino acid metabolism of CD8^(+)T cells,we examined the effects of 0.3 T moderate static magnetic field on the concentration levels of amino acids of stimulated CD8^(+)T cells by mass spectrographic analysis.The results showed that the concentrations of 4 types of amino acids outstanding of 42 amino acids were obviously increased under static magnetic field exposure,including Tau,Glu,1MHis and Asa.The next in vitro culturing of CD8^(+)T cells by adding amino acids in medium revealed that the secretion level of Granzyme B in CD8^(+)T cells were elevated in case of adding 1μmol/L Tau or Glu.To explore the related molecular mechanisms,m RNA expression levels of nutrition metabolism related genes analyzed by RT-PCR and surface marker expression of nutrition receptors analyzed by FACS were performed.The results indicated that moderate static magnetic field enhanced the expression levels of nutrition receptor CD98 and CD71.It’s possible that moderated static magnetic field influences the metabolism of amino acids in CD8^(+)T cells by upregulating related nutrition receptor.