高效液相色谱-电感耦合等离子体质谱法测定畜禽食品中十种砷形态

Determination of ten arsenic compounds in poultry food by high performance liquid chromatography-inductively coupled plasma mass spectrometry

目的利用高效液相色谱-电感耦合等离子体质谱(HPLC-ICP-MS)联用技术,建立畜禽食品中砷形态化合物的分析方法。方法以10%甲醇和1%硝酸混合液为提取液,对样品进行微波辅助萃取,选取DIonexIonPac As7(4 mm×250 mm)阴离子交换色谱柱,采用0.5~200 mmol/L碳酸铵进行梯度洗脱,测定10种砷形态化合物。结果10种砷形态化合物在1~20μg/L浓度范围内线性良好,相关系数(r)>0.9990,方法检出限为0.24~0.80μg/kg,精密度的相对标准偏差(RSD)为1.0%~5.7%,鸡肉和牛肉基质3个浓度水平加标回收率在83.77%~114.21%之间。结论本方法具有灵敏度高、前处理简便、重现性及准确性好等优势,适用于畜禽食品中10种砷形态化合物的测定。

Objective performance liquid chromatography-inductively coupled plasma mass spectrometry(HPLC-ICP-MS).Methods sample was microwave assisted extracted by 10%methanol and 1%nitric acid.Ten arsenic compounds were separated by DIonexIonPac As7(4 mm×250 mm)anion exchange column with 0.5-200 mmol/L ammonium carbonate for gradient elution.Results than 0.9990,and the detection limits of the method were between 0.24-0.80μg/kg.The relative standard deviations(RSDs)of precision ranged from 1.0%to 5.7%.The recoveries were 83.77%-114.21%in chicken and beef substrate,spiked with the three levels.Conclusion accuracy,the method was suitable for the determination of ten arsenic compounds in poultry food.