转BpCCR1基因白桦生长适应性分析及优良株系选择

Growth adaptability analysis of BpCCR1 transgenic Betula platyphylla and selection of elite lines

【目的】客观评估7年生转BpCCR1白桦在3个试验地点的生长适应性与稳定性,筛选优良转基因株系,为环境释放及生产性试验提供指导。【方法】以21个转BpCCR1白桦株系及1个野生型白桦株系(WT)为研究对象,测定其在帽儿山、石道河林场、生态实验林场3个试验地点的树高、胸径及材积生长,用AMMI模型分析参试株系与环境的互作效应、遗传稳定性和生长适应性。【结果】方差分析结果显示:树高生长在株系间、地点间以及株系与地点的交互作用上的差异达到极显著水平(P<0.01)。以各试验点参试株系单株材积均值加上标准差为优良转基因株系选择标准,分别选择出各造林地点的最优株系。帽儿山试验点的入选优良株系是FC18、FC29和FC40,材积分别高于群体均值的72.99%、51.28%和64.39%;石道河林场的入选优良株系是FC7和FC33,材积分别高于群体均值的73.98%和81.51%;生态实验林场的入选优良株系是FC28和FC29,分别高于群体均值的69.89%和73.57%。采用AMMI模型对参试株系的树高性状进行稳定性评价,在帽儿山实验林场和生态实验林场生长稳定性较好的株系是:WT、FC11、FC16、FC19、FC28、FC29、FC30、FC31、C4;在石道河林场生长稳定性好的株系是:FC2、FC7、FC13、FC14、FC18、FC27、FC33、FC40、FC41、C6、C10、C17、C19。根据第一主成分分量IPC1及相对稳定性参数Dg值,对22个参试株系进行稳定性评价,结果显示:FC14、FC29、C19、FC27、FC41、WT、FC13、C4、C10、C6、FC16、FC7、FC28、FC40、C17、FC31、FC18属于高产稳产型株系;FC2、FC11、FC19、FC33属于高产非稳产型株系;FC30属于低产稳产型株系。【结论】转BpCCR1正义链及转反义链均能促进白桦树高生长,根据生长性状,选出17个株系作为在帽儿山林场、石道河林场及生态实验林场生长适应性好的高产株系。

[Objective]This study aims to conduct a joint analysis of the growth adaptability and stability of the 7-year-old BpCCR1 transgenic birch in 3 test locations,screen high-quality transgenic lines,provide guidance for environmental release and production experiments.[Method]The tree height,diameter at breast height(DBH)and volume at Maoershan Experimental Forest Farm,Shidaohe Forest Farm and Experimental Forest Farm of northeastern China were measured with 21 BpCCR1 transgenic white birch and 1 wild-type white birch(WT)as the research objects.The AMMI model was used to carry out the interaction effects between the tested lines and the environment,genetic stability and growth adaptability.[Result]The analysis of variance showed that the differences in height between lines,locations,and interactions between lines and locations were extremely significant(P<0.01).The best lines for each test location were selected by taking the average volume plus the standard deviation of the tested plants at each test site as the selection criteria for elite transgenic lines.The elite lines selected from the Maoershan Experimental Forest Farm were FC18,FC29,FC40,whose volumes were 72.99%,51.28%,64.39%higher than the population average,respectively.The elite lines selected from the Shidaohe Forest Farm were FC7 and FC33,whose volumes were 73.98%,81.51%higher than the population average,respectively.The elite lines selected from the Ecological Experiment Forest Farm were FC28 and FC29,whose volumes were 69.89%and 73.57%higher than the population average,respectively.The AMMI model was used to evaluate the stability of the height of the tested lines.The lines with better growth stability in the Maoershan Experimental Forest Farm and Ecological Experimental Forest Farm were WT,FC11,FC16,FC19,FC28,FC29,FC30,FC31,C4.Lines with better growth stability in Shidaohe Forest Farm were FC2,FC7,FC13,FC14,FC18,FC27,FC33,FC40,FC41,C6,C10,C17,C19.22 test lines were evaluated for stability according to IPC1 and the Dg value.The results showed that FC14,FC29,C19,FC27,FC41,WT,FC13,C4,C10,C6,FC16,FC7,FC28,FC40,C17,FC31,FC18 were high-yielding and stable-yielding lines;FC2,FC11,FC19,and FC33 were high-yielding and unstable-yielding lines;FC30 was a low-yielding and stable-yielding line.[Conclusion]Both the sense and antisense BpCCR1 transgenes promot tree height of birch.17 lines were selected based on their growth traits as high-yielding lines with good growth adaptation in the Maoershan Experimental Forest Farm,Shidaohe Forest Farm and Experimental Forest Farm.