TRIM58与OVOL1蛋白在结直肠癌组织中的表达及意义

Expression of TRIM58 and OVOL1 protein in colorectal cancer and its significance

目的:探讨三基序蛋白58(TRIM58)与OVOL1蛋白在结直肠癌组织中的表达及对临床预后的意义。方法:选取2016年11月—2017年10月经抚顺市中心医院确诊并进行治疗的62例结直肠癌患者,收集其癌组织标本及对应的癌旁组织标本。采用免疫组织化学染色法检测癌组织与癌旁组织中TRIM58与OVOL1蛋白表达水平。采用χ^(2)检验和Cox回归分析分别探讨TRIM58与OVOL1蛋白表达与结直肠癌患者临床病理特征及预后的关系。结果:结直肠癌组织中TRIM58、OVOL1蛋白高表达率均明显低于癌旁组织(32.26%比61.29%;35.48%比59.68%;P<0.001)。不同年龄、性别、肿瘤部位、分化程度及肿瘤直径结直肠癌患者TRIM58、OVOL1高低表达率差异无统计学意义(P>0.05);但结直肠癌不同TNM分期、是否淋巴结转移患者TRIM58、OVOL1高低表达率差异有统计学意义(P<0.05),且TNM分期越高、出现淋巴结转移的结直肠癌组织中的TRIM58、OVOL1表达明显降低(P<0.05)。TRIM58蛋白高表达的结直肠癌患者中位生存期(35个月)明显高于TRIM58蛋白低表达患者(25个月),OVOL1蛋白高表达的结直肠癌患者中位生存期(36个月)明显高于OVOL1蛋白低表达患者(24个月),差异均有统计学意义(P<0.05)。单因素分析得出,TNMⅢ~Ⅳ期、淋巴结转移、TRIM58低表达、OVOL1低表达结直肠癌患者的生存时间均缩短(P<0.05);Cox多因素回归分析得出:TNMⅢ~Ⅳ期、淋巴结转移、TRIM58低表达、OVOL1低表达均为结直肠癌预后的危险因素(P<0.05)。结论:TRIM58、OVOL1蛋白在结直肠癌组织中均呈低表达,TRIM58、OVOL1蛋白表达水平与患者临床病理特征显著相关,TRIM58、OVOL1蛋白低表达的结直肠癌患者预后较差。

Objective:To investigate the expression of Three-motif protein 58(TRIM58)and OVOL1 protein in colorectal cancer and its clinical prognostic significance.Methods:A total of 62 patients with colorectal cancer who were diagnosed and treated in Fushun Central Hospital from November 2016 to October 2017 were selected,and their cancer tissue specimens and corresponding paracancerous tissue specimens were collected.The expression levels of TRIM58 and OVOL1 protein in cancer tissues and adjacent tissues were detected by immunohistochemical staining.The relationship between TRIM58,OVOL1 expression and clinicopathological features and prognosis of colorectal cancer patients were investigated by chi square test and Cox regression analysis.Results:The high expression rates of TRIM58 and OVOL1 proteins in colorectal cancer tissues were significantly lower than those in adjacent tissues(32.26%vs 61.29%;35.48%vs 59.68%,P<0.001).The expression of TRIM58 and OVOL1 had no significant difference in colorectal cancer patients with different age,gender,tumor location,differentiation degree and tumor diameter(P>0.05),but they were significantly correlated with tumor TNM and lymph node metastasis(P<0.05),and the higher TNM stage of tumor or lymph node metastasis,the lower the expression of TRIM58 and OVOL1 in colorectal tissues(P<0.05).The median survival time of TRIM58 protein high expression and low expression expression was 35 months and 25 months,respectively,and the median survival time of OVOL1 protein high expression and low expression expression was 36 months and 24 months respectively,and the difference was statistically significant(P<0.05).Univariate analysis showed that the survival time of colorectal cancer patients with TNMⅢ~Ⅳ,lymph node metastasis,TRIM58 low expression,and OVOL1 low expression were all shortened(P<0.05).Cox multivariate regression analysis showed:TNMⅢ~Ⅳ,Lymph node metastasis,low expression of TRIM58,and low expression of OVOL1 were all independent risk factors affecting the prognosis of colorectal cancer(P<0.05).Conclusion:TRIM58 and OVOL1 proteins are low in colorectal cancer tissues,and their expression levels are significantly related to changes in the clinical pathological characteristics of patients.Colorectal cancer patients with low expression of TRIM58 or OVOL1 proteins have a poor prognosis.