重离子突变藻株筛选及沼液处理性能研究

Study on Screening of Heavy Ion Mutant Microalgae Strains and Treatment Performance of Biogas Sludge

为了提高微藻在高浓度氮磷污水中的处理性能,利用重离子束辐照诱变普通小球藻CK-1#,选育得到突变藻株HM-3#。结果表明,HM-3#在模拟污水中生长良好,污染物去除效果相比初始藻株CK-1#提高明显。进一步考察HM-3#对猪场稀释沼液的处理性能,处理时间缩短1倍,生物量、光合色素和油脂含量可达到模拟污水中的1.50、1.57和2.19倍;NH_(4)^(+)-N、TP和COD去除速率保持稳定,甚至优于在模拟污水中的结果,其值分别为22.44、2.43、47.84 mg/(L·d)。综上,本研究通过重离子束辐照诱变筛选到一株适用于高浓度氮磷污水处理的优良突变藻株HM-3#,并于稀释沼液中验证该藻株具有良好的生长速率与高效的氮磷去除性能,为实现污水资源化利用提供了新的解决思路。

In order to improve the treatment performance of microalgae in high-concentration nitrogen and phosphorus wastewater,the Chlorella CK-1#was mutated by heavy ion beam irradiation,and the mutant microalgae strain HM-3#was bred.The results showed that HM-3#grew well in the simulated sewage,and the pollutant removal efficiency was significantly improved compared with the original CK-1#.Therefore,the treatment performance of HM-3#on diluted piggery digestate was further investigated,and the biomass,photosynthetic pigment accumulation and oil accumulation could reach 1.50,1.57 and 2.19 times of simulated sewage by shorting the treatment time by 1 times.The removal rates of NH_(4)^(+)-N,TP and COD remained stable and even better than those in simulated wastewater,which were 22.44,2.43 and 47.84 mg/(L·d),respectively.To sum up,this study screened the excellent mutant microalgae strain HM-3#suitable for high-concentration nitrogen and phosphorus wastewater treatment through heavy ion beam irradiation mutation,and verified that this microalgae strain had a good growth rate and efficient nitrogen and phosphorus removal performance in diluted piggery digestate,providing a new solution for the realization of wastewater resource utilization.