臭氧自体血回输疗法对急性期带状疱疹的疗效及T淋巴细胞亚群的影响

Clinical efficacy of ozonated autohemotherapy on the patients with acute herpes zoster and its influences on T-lymphocyte subsets

目的观察臭氧自体血回输疗法对急性期带状疱疹临床疗效及细胞免疫功能的影响。方法收集2018年3月至2018年12月泰安市中心医院疼痛科住院治疗急性期带状疱疹患者60例,采用随机数字法分为3组(n=20):椎旁神经射频治疗组(A组)、联合臭氧自血治疗3 d组(B组)、联合臭氧自血治疗5 d组(C组)。A组采用加巴喷丁、氨酚双氢可待因口服,并行椎旁脉冲射频治疗1次;B组在A组基础上,给予25μg/ml臭氧自体血回输3 d,1次/d;C组在A组基础上给予25μg/ml臭氧自体血回输5 d,1次/d。分别记录治疗前、治疗后第4、6日时患者VAS评分,采用简明疼痛量表(brief pain inventory,BPI)评估患者生活质量相关指标,监测外周血T淋巴细胞亚群中CD3^(+)、CD4^(+)、CD8^(+)的百分比及CD4^(+)/CD8^(+)比值。结果3组患者治疗后第4、6日的VAS评分及BPI评分与治疗前相比均下降,差异具有统计学意义(P<0.001);CD3^(+)、CD4^(+)淋巴细胞百分比及CD4^(+)/CD8^(+)比值均较治疗前升高,CD8^(+)百分比均较治疗前降低,差异均具有统计学意义(P均<0.001);C组治疗后第6日VAS评分(0.48±0.16)分,较B组(1.41±0.42)分降低,差异具有统计学意义(P<0.001)。C组治疗后第6日CD3^(+)、CD4^(+)淋巴细胞百分比及CD4^(+)/CD8^(+)比值均较B组升高,CD8^(+)的百分比均较B组降低,差异均有统计学意义(P均<0.001)。结论臭氧自体血回输疗法可以显著减轻带状疱疹患者的疼痛、改善患者的生活质量,并明显改善患者的免疫功能。

Objective To observe the clinical efficacy of ozonated autohemotherapy on acute herpes zoster and its influences on immune function.Methods Sixty patients with herpes zoster were randomly divided into 3 groups(n=20 each):paravertebral nerve radiofrequency therapy group(group A),combined ozonated autohemotherapy for 3 days group(group B)and combined ozonated autohemotherapy for 5 days group(group C).Paracetamol and dihydrocodeine were taken orally and paravertebral nerve radiofrequency therapy was performed once in group A.On the basis of group A,patients were given ozonated autohemotherapy with ozone 25μg/ml in group B once a day for 3 days,while patients were given ozonated autohemotherapy with ozone 25μg/ml in group C once a day for 5 days.VAS was recorded before the treatment,and at the 4th and 6th days after the treatment,and a brief pain inventory(BPI)was used to evaluate the quality of life in the patients.The percentages of CD3^(+),CD4^(+),CD8^(+)and the ratio of CD4^(+)/CD8^(+)were monitored in the peripheral blood T-lymphocyte subsets.Results VAS and BPI were all decreased in the three groups at the 4th and 6th days after the treatment,compared with the pretreatment,with significant differences(all P<0.001).The percentages of CD3^(+),CD4^(+)and CD4^(+)/CD8^(+)were all increased compared with the pretreatment,and the percentages of CD8^(+)were all decreased compared with pretreatment,with statistically significant differences(all P<0.001).VAS was(0.48±0.16)in group C at the 6th day after the treatment,which was lower than that in group B(1.41±0.42)(P<0.001).In group C,the percentages of CD3^(+)and CD4^(+)and the ratio of CD4^(+)/CD8^(+)were all higher than those in group B at the 6th day after the treatment.And the percentages of CD8^(+)were all lower than those in group B(all P<0.001).Conclusion Ozonated autohemotherapy can significantly reduce the pain of patients,improve the quality of life,and significantly improve the immune function of patients with acute herpes zoster.