蒲公英黄酮对ICR小鼠体内抗氧化酶活性及相关基因表达的影响

Effects of Dandelion Flavonoids on Antioxidant Enzyme Activity and Related Gene Expression in ICR Mice

为探讨蒲公英黄酮对ICR(institute of cancer research)小鼠体内抗氧化酶活性及相关基因表达的影响,将50只5周龄SPF级ICR雄性小鼠(22 g±2 g)按体质量随机分为5组:空白组、对照组、高浓度蒲公英黄酮组(100 mg·kg^(-1)·d^(-1))、中浓度蒲公英黄酮组(50 mg·kg^(-1)·d^(-1))、低浓度蒲公英黄酮组(25 mg·kg^(-1)·d^(-1))。各实验组每天定时灌胃不同浓度蒲公英黄酮1次,空白组灌胃等量的生理盐水,对照组灌胃等量二甲基亚砜(DMSO);连续4周,利用相关试剂盒测定小鼠血清和肝脏组织内超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)的活性,总抗氧化能力(total antioxidant capacity,T-AOC),以及丙二醛(malondialdehyde,MDA)的含量;利用RT-qPCR方法检测小鼠肝脏组织中抗氧化基因SOD-1、SOD-2、GPX-1、GPX-4的mRNA相对表达量。结果显示,蒲公英黄酮能显著提高小鼠血清和肝脏组织中SOD、GSH-PX、T-AOC的活性(P<0.05),显著降低MDA的含量(P<0.05),显著上调抗氧化基因SOD-1、SOD-2、GPX-1、GPX-4的mRNA的表达量。研究表明,蒲公英黄酮能提高小鼠的抗氧化能力,具有抗衰老作用。

To investigate the effect of dandelion flavonoids on antioxidant enzyme activity and gene expression in mice,fifty 5-week-old SPF ICR male mice(22 g±2 g)were divided into a while the control group,control group,high concentration of dandelion flavonoid group(100 mg·kg^(-1)·d^(-1)),medium concentration of dandelion flavonoid group(50 mg·kg^(-1)·d^(-1)),low concentration of dandelion flavonoid group(25 mg·kg^(-1)·d^(-1)),test group was given different concentrations of dandelion flavonoid once a day by regular gavage,while the control group was given the same amount of normal saline for 4 weeks,control group was given the same amount of normal DMSO for 4 weeks.The activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX),total antioxidant capacity(T-AOC),and malondialdehyde(MDA)content were determined in the serum and liver tissues of mice,and the relative mRNA expression of SOD-1,SOD-2,GPX-1,GPX-4 in liver tissues of mice were determined by RT-qPCR.The results show that dandelion flavonoids can significantly increase the activities of SOD,GSH-PX,T-AOC in mouse serum and liver tissue increased significantly(P<0.05),the content of MDA reduced significantly(P<0.05),and the expression of SOD-1,SOD-2,GPX-1,GPX-4 mRNA increased significantly.Research shows,dandelion flavonoids can improve the antioxidant capacity of mice and have anti-aging effects.