紫花苜蓿ARR基因克隆及转化

Cloning and Transformation of ARR Gene in Medicago sativa

细胞分裂素响应调节因子ARRs(Arabidopsis response regulators)在细胞分裂素的信号转导过程中发挥极为重要的作用。紫花苜蓿(Medicago sativa)作为一种重要的多年生优质牧草植物,在我国草产业中地位显著,越来越受到科研工作者的关注。为研究紫花苜蓿ARR基因功能,采用RT-PCR技术成功克隆ARR基因。采用农杆菌(Agrobacterium tumefacien)转化法将成功构建的3302-ARR-3Flag植物表达载体导入野生型紫花苜蓿,并成功获得9株紫花苜蓿转基因植株。与野生型相比,转基因紫花苜蓿分枝数多,复叶数目异常,为深入研究ARR基因功能提供原始实验材料。

Arabidopsis response regulators(ARRs)play extremely important roles in cytokinin signaling transduction.As an important forage plant,alfalfa(Medicago sativa)has a prominent position in grass industry and is becoming the research focus of scientific researchers.To investigate the function of the ARR gene,the ARR gene was successfully isolated using RT-PCR technology.The 3302-ARR-3 Flag plant expression vector was introduced into wild-type alfalfa by Agrobacterium tumefacien-mediated transformation,and nine transgenic plants of alfalfa were successfully obtained.Compared with the wild alfalfa type,the transgenic alfalfa had more branches and abnormal number of compound leaves,which provided the original experimental materials for further study of ARR gene function.