CD8^(+)T淋巴细胞在成人传染性单核细胞增多症患者中的表达分析

Analysis of expression of CD8^(+)T lymphocytes in adult infectious mononucleosis

目的:检测EB病毒(EBV)感染所致成人传染性单核细胞增多症(IM)患者外周血淋巴细胞亚群和血浆EBV DNA载量,分析EBV载量与淋巴细胞亚群的相关性。方法:收集2016至2021年确诊的成人IM患者318例作为IM组,选择同期体检的健康成人31例作为对照组。采用多参数流式细胞术检测外周血各淋巴细胞亚群占总淋巴细胞百分比,实时荧光定量PCR测量血浆中的EBV DNA载量。结果:IM组CD8^(+)CD3^(+)(53.39±8.02)%和CD8弱阳性CD3^(+)(12.17±8.52)%比例明显增高,与对照组比较差异有统计学意义(P<0.001);CD4^(+)CD3^(+)(30.7±9.83)%和CD4^(+)/CD8^(+)(0.60±0.25)明显降低,与对照组比较差异有统计学意义(P<0.05);IM组CD3^(+)、CD16^(+)CD56^(+)CD3^(+)比例和对照组比较差异均无统计学意义(P>0.05);IM组EBV DNA载量与CD8^(+)CD3^(+)和CD8弱阳性CD3^(+)比例呈正相关(P<0.05),与CD3^(+)、CD4^(+)CD3^(+)、CD16^(+)CD56^(+)CD3^(+)比例无相关性(P>0.05)。结论:成人IM患者外周血CD8^(+)T淋巴细胞和CD8弱阳性T淋巴细胞显著增多,EBV DNA载量与CD8^(+)T淋巴细胞和CD8弱阳性T淋巴细胞呈正相关,综合淋巴细胞亚群检测结果与血浆EBV DNA载量有助于疾病的诊断。

Objective:To detect the peripheral blood lymphocyte subsets and plasma EBV DNA load in adult infectious mononucleosis patients infected by EBV virus,and analyze the correlation between EB virus load and lymphocyte subsets.Methods:A total of 318 adult infectious mononucleosis patients diagnosed in our hospital from 2016 to 2021 were collected as the experimental group(IM group),and 31 healthy adults who underwent physical examination during the same period were selected as the control group.Multi-parameter flow cytometry was used to detect the percentage of lymphocyte subsets in peripheral blood to total lymphocytes,and real-time fluorescence quantitative PCR was used to measure EBV DNA load in plasma.Results:The proportion of CD8^(+)CD3^(+)(53.39±8.02)%and CD8 weakly positive CD3^(+)(12.17±8.52)%in IM group were significantly higher than those in control group(P<0.001).CD4^(+)CD3^(+)(30.7±9.83)%and CD4^(+)/CD8^(+)(0.60±0.25)were significantly lower than those in the control group(P<0.05).There was no significant difference in the ratio of IM CD3^(+),CD16^(+)CD56^(+)CD3^(+)between IM group and control group.In IM group,EBV DNA load was positively correlated with the proportion of CD8^(+)CD3^(+)and CD8 weakly positive CD3^(+)(P<0.05),but not correlated with the proportion of CD3^(+),CD4^(+)CD3^(+)and CD16^(+)CD56^(+)CD3^(+)(P>0.05).Conclusion:CD8^(+)T lymphocytes and CD8 weakly positive T lymphocytes in peripheral blood of adult IM patients significantly increased,and EBV DNA load was positively correlated with the proportion of CD8^(+)T lymphocytes and CD8 weakly positive T lymphocytes.Comprehensive detection results of lymphocyte subsets and plasma EBV DNA load may be helpful for diagnosis of the disease.