间充质干细胞治疗变应性鼻炎:一项基于动物实验的Meta分析

Mesenchymal stem cells for allergic rhinitis:a meta-analysis based on animal experiments

目的:变应性鼻炎是一种全球性蔓延过敏性疾病,严重影响人们的生活质量,且临床上治疗效果往往不佳。通过系统评价间充质干细胞对变应性鼻炎动物模型的疗效,希望为临床医师提供启发。方法:检索中国知网、万方、维普、中国生物医学文献数据库和Embase、Cochrane library、PubMed、Web of Science中有关间充质干细胞治疗变应性鼻炎小鼠的相关文章。检索时间为各数据库建立至2022-03-01,分别由2名研究者独立提取数据,采用SYRCLE动物实验偏倚风险评估表进行文献质量评价。采用stata 16.0进行Meta分析。结果:共纳入6篇随机对照动物实验,包括152只小鼠,干细胞组小鼠51只,空白对照组51只,动物模型对照组小鼠50只。Meta分析结果显示:干细胞组喷嚏次数明显少于动物模型对照组[SMD=-9.83,95%CI(-17.45,-2.22),P=0.011];干细胞组揉鼻次数明显少于动物模型对照组[SMD=-13.27,95%CI(-25.04,-1.49),P=0.027];干细胞组血清白细胞介素4水平明显低于动物模型对照组[SMD=-8.23,95%CI(-11.28,-5.17),P=0];干细胞组血清白细胞介素6水平明显低于动物模型对照组[SMD=-4.27,95%CI(-5.43,-3.10),P=0.035];干细胞组脾脏白细胞介素10水平明显高于动物模型对照组[SMD=25.63,95%CI(17.92,33.33),P=0];干细胞组脾脏白细胞介素6水平明显低于动物模型对照组[SMD=-40.64,95%CI(-80.16,-1.13),P=0]。结论:根据纳入的动物实验表明,干细胞有助于改善变应性鼻炎的症状,降低血清白细胞介素4、白细胞介素6水平和脾脏白细胞介素6水平,升高脾脏中白细胞介素10水平,干细胞在变应性鼻炎中起着重要的作用,期待今后能够开展临床试验对其安全性和有效性进行详细评估。

OBJECTIVE:Allergic rhinitis is a globally prevalent allergic disease that seriously affects people's quality of life and often has a poor clinical treatment effect.The effects of mesenchymal stem cells on allergic rhinitis animal models were systematically evaluated,hoping to provide inspiration for clinicians.METHODS:Articles related to the treatment of allergic rhinitis mice with mesenchymal stem cells were retrieved from CNKI,Wanfang,VIP,Chinese biomedical literature database,Embase,Cochrane Library,PubMed,and Web of Science.The retrieval time was from the establishment of the database to March 1,2022.Data were independently extracted by two researchers.SYRCLE Animal Experiment Bias Risk Assessment table was used to evaluate the literature quality.Stata 16.0 was used for meta-analysis.RESULTS:A total of 152 mice were included in 6 randomized controlled animal experiments,including 51 mice in stem cell group,51 mice in blank control group,and 50 mice in animal model control group.The meta-analysis results demonstrated that the number of sneezes in stem cell group was significantly less than that in the animal model control group[SMD=-9.83,95%CI(-17.45,-2.22),P=0.011].The number of nose rubbing in stem cell group was significantly less than that in the animal model control group[SMD=-13.27,95%CI(-25.04,-1.49),P=0.027].Serum interleukin-4 level in stem cell group was significantly smaller than that in the animal model control group[SMD=-8.23,95%CI(-11.28,-5.17),P=0].Serum interleukin-6 level in stem cell group was significantly smaller than that in the animal model control group[SMD=-4.27,95%CI(-5.43,-3.10),P=0.035].Interleukin-10 level in the spleen in stem cell group was significantly higher than that in the animal model control group[SMD=25.63,95%CI(17.92,33.33),P=0].Interleukin-6 level in the spleen in stem cell group was significantly lower than that in the animal model control group[SMD=-40.64,95%CI(-80.16,-1.13),P=0].CONCLUSION:According to the included animal experiments,stem cells help to improve the symptoms of allergic rhinitis,decrease serum interleukin-4 and interleukin-6 levels and spleen interleukin-6 level,and increase spleen levels of interleukin-10.Stem cells play an important role in allergic rhinitis,and clinical trials are expected to carefully evaluate their safety and efficacy in the future.