一株红树植物内生真菌Pseudallescheria boydii L32的代谢产物

The metabolites of a mangrove endophytic fungus Pseudallescheria boydii L32

研究了一株红树林植物苦槛蓝Myoporum bontioides A.Gray内生真菌Pseudallescheria boydii L32代谢产物,用现代色谱技术从发酵产物中分离单体,波谱分析鉴定结构,二倍稀释法测试抗菌活性。分离鉴定了2,2′-oxybis(1,4)-di-tert-butylbenzene(1),livistone B(2),simplicildone A(3),simplicildone B(4),botryorhodine B(5),(-)-regiolone(6),citreoanthrasteroid B(7),豆甾-4-烯-3-酮(8),丁二酸(9)和草酸(10)等10个代谢产物。其中代谢物1,3,4,5,7,8为首次从Pseudallescheria属分离到。代谢物1,4,5,6对O6血清型大肠杆菌,代谢物1,5对O78血清型大肠杆菌显示抑菌活性,最小抑菌质量浓度分别为50,12.5,25,100,100,100μg/mL。

The metabolites of an endophytic fungus Pseudallescheria boydii L32 derived from the mangrove plant Myoporum bontioides A.Gray were studied.The monomeric compounds were isolated from the fermentation product by the modern chromatography technology.The structures were determined by spectral analysis.The antibacterial activity was tested via the dilution method.Ten metabolites were purified including 2,2′-oxybis(1,4)-di-tert-butylbenzene(1),livistone B(2),simplicildone A(3),simplicildone B(4),botryorhodine B(5),(‒)-regiolone(6),citreoanthrasteroid B(7),stigmast-4-en-3-one(8),succinic acid(9)and oxalic acid(10).Compounds 1,3,4,5,7 and 8 were isolated from Pseudallescheria sp.for the first time.Compounds 1,4,5 and 6 showed antibacterial activity against Escherichia coli with O6 serotype and compounds 1 and 5 showed antibacterial activity against E.coli with O78 serotype with MIC values of 50,12.5,25,100,100,100μg/mL,respectively.