双参粗提组分对高糖诱导大鼠肾小球系膜细胞的影响

Effects of Crude Extraction Fractions from Triplostegia Glandulifera on High Glucose-induced Rat Glomerular Mesangial Cells

研究药用植物双参对高糖诱导大鼠肾小球系膜细胞异常增殖的影响,本实验对双参进行水提,水提液经脱蛋白、脱色素以及95%乙醇醇沉,得到双参粗提组分。检测该组分对高糖诱导大鼠肾小球系膜细胞异常增殖的抑制作用,以及其对高糖模型细胞中氧化应激指标超氧化物歧化酶(SOD)和丙二醛(MDA)含量的影响。结果表明,该双参粗提组分可明显抑制高糖诱导大鼠肾小球系膜细胞的异常增殖,同时可显著提高模型细胞中SOD的活力,降低MDA的含量,提示其对高糖诱导大鼠肾小球系膜细胞具有保护作用。

To study the effect of the medical plant Triplostegia glandulifera on the abnormal proliferation of rat glomerular mesangial cells induced by high glucose,T.glandulifera was extracted with water in this experiment.The crude extraction fractions from T.glandulifera were extracted by alcohol precipitation.The inhibitory effect of the crude extraction fractions on the abnormal proliferation of rat glomerular mesangial cells induced by high glucose,and its activities or contents of oxidative stress indicators(SOD,MDA) in the model cells were detected.The results showed that the crude extraction fractions of T.glandulifera could significantly inhibit the abnormal proliferation of rat glomerular mesangial cells induced by high glucose.Morover,it could significantly increase the activities of SOD and reduce the contents of MDA in the model cells.Therefore,the fractions had a protective effect on high glucose-induced rat glomerular mesangial cells.