硒化氨基多糖对黑鲷脾脏代谢物的影响

Effects of Selenized Glycosaminoglycan on the Spleen Metabolites of Acathopagrus schlegelii

通过研究饲喂硒化氨基多糖后黑鲷脾脏代谢物的变化,探讨其免疫调节机制。对照组未添加硒化氨基多糖,试验组饲喂添加硒化氨基多糖的饲料,采用超高效液相色谱-飞行时间质谱联用技术(UPLC-QTOF-MS),结合XCMS^(plus)软件、高分辨二级数据库对副溶血弧菌攻毒48和96 h时黑鲷脾脏内源性代谢物进行了非靶向代谢组学分析,筛选出潜在的脾脏内源性生物标志物。通过MetaboAnalyst 4.0网站进行相关代谢通路分析,揭示硒化氨基多糖调节脾脏免疫的潜在机制。结果表明,黑鲷脾脏在副溶血弧菌攻毒48和96 h时,分别有存在显著差异的31和36种潜在生物标志物,指向了8和10条代谢通路(P<0.05)。硒化氨基多糖通过多条代谢通路增强了黑鲷脾脏的自身免疫机能。该研究结果为阐明硒化氨基多糖的免疫增强机制和合理开发免疫增强剂提供了科学依据。

The changes of spleen metabolites in Acathopagrus schlegelii after feeding selenized glycosaminoglycan were studied,and the immunomodulatory mechanism was discussed.The selenized glycosaminoglycan was not added in control group,the feed with adding selenized glycosaminoglycan was fed in experimental group.Using UPLC-QTOF-MS,combined with XCMS^(plus) software and high-resolution secondary database,untargeted metabolomic analysis was made on the endogenous metabolites of A.schlegelii’s spleen challenged by V.parahaemolyticus 48 and 96 h,potential endogenous biomarkers of spleen were screened out.The related metabolic pathways were analyzed on MetaboAnalyst 4.0 website.The potential mechanism of selenized glycosaminoglycan in regulating the immunity of spleen was revealed.The results showed that 31 and 36 potential biomarkers were found in the spleen of A.schlegelii challenged by V.parahaemolyticus 48 and 96 h respectively,which were involved in 9 and 10 metabolic pathways(P<0.05).The immune function of A.schlegelii spleen was enhanced by selenized glycosaminoglycan through multiple metabolic pathways.The study results provided the scientific basis for elucidating the immunoenhancement mechanism of selenized glycosaminoglycan and rational development of immune enhancers.