内部沸腾法提取骨碎补多糖及其抗氧化活性研究

Study on Extraction of Rhizoma Drynariae Polysaccharide and Its Antioxidant Activity by Internal Boiling Method

以骨碎补为试验材料,采用内部沸腾法来提取骨碎补中的多糖,并且测定骨碎补里提取出来的多糖的抗氧化活性,通过设置单因素试验和4因素3水平的正交试验,研究乙醇解吸液体积分数、乙醇解吸时间、水浴提取温度、水浴提取时间对骨碎补多糖提取率的影响。结果表明,提取骨碎补中多糖的最佳条件为乙醇解吸液体积分数50%、解吸时间15 min、水浴提取温度80℃、水浴提取时间4 min,在这个条件下的骨碎补多糖提取率为10.90%。当骨碎补多糖质量浓度为0.5%时,其对DPPH自由基的清除率达74.8%,对羟基自由基的清除率达78.3%。

Taking Rhizoma Drynariae as the test material,the internal boiling method was used to extract the polysaccharides in Rhizoma Drynariae,and the antioxidant activity of the polysaccharides extracted from Rhizoma Drynariae was determined.The effects of ethanol desorption liquid fraction,ethanol desorption time,water-bath extraction temperature and water-bath extraction time on the extraction rate of Rhizoma Drynariae were studied by setting up single factor experiment and orthogonal experiment with 4 factors and 3 levels.The results showed that the optimal conditions for extracting polysaccharides from Rhizoma Drynariae were 50%ethanol desorption liquid fraction,15 min desorption time,80℃water-bath extraction temperature,and 4 min water-bath extraction time.Under these conditions,the extraction rate of Rhizoma Drynariae polysaccharide was 10.90%.When the mass concentration of drynaria polysaccharide was 0.5%,its scavenging rate for DPPH free radicals reached 74.8%,and the scavenging rate for hydroxyl radicals reached 78.3%.