基于KEGG通路分析紫花苜蓿的冷适应过程

Analyzing Cold Acclimation Process of Alfalfa Based on KEGG Pathway

低温是影响我国苜蓿(Medicago stavia L.)种植推广的限制性因素,且苜蓿对低温的冷适应机制亦不完全清楚。本研究利用高通量测序技术,测定‘肇东’和‘WL440HQ’苜蓿在冷适应前后的基因表达。筛选差异表达基因后,分析与冷适应低温环境和苜蓿抗寒性提高有关的生物通路以及造成冷适应后苜蓿抗寒性出现差异的可能原因。结果表明:细胞信号转导和物质代谢(包括糖、蛋白质和脂等)等通路,不仅是苜蓿对冷适应低温环境的响应,还可能与其抗寒性的提高有关,如植物激素信号转导、淀粉和蔗糖代谢和植物MAPK信号通路等。冷适应后苜蓿抗寒性出现差异是多种生物通路共同作用下的结果,其中主要涉及物质代谢(包括脂肪酸和氨基酸等)和能量代谢等通路,特别是氨基酸和脂肪酸代谢。本研究将为苜蓿抗寒机制的深入研究提供数据支持。

Low temperature is a limiting factor affecting the planting and promotion of alfalfa in China,and the adaptation mechanism to low temperature is still not fully understood.In this study,the gene expression of‘ZhaoDong’and‘WL440HQ’alfalfa before and after cold acclimation was determined by high-throughput sequencing technology.After screening differentially expressed genes,the biological pathways related to cold adaptation and freezing tolerance of alfalfa were analyzed to explore the possible reasons for the difference in freezing tolerance of alfalfa after cold acclimation.The results showed that cellular signal transduction and substance metabolism(including sugar,protein,lipid and so on)were not only the response of alfalfa to cold acclimation,but also related to the improvement of freezing tolerance,such as plant hormone signal transduction,starch and sucrose metabolism and MAPK signaling pathway-plant.The difference in freezing tolerance of alfalfa after cold acclimation was the result of the joint action of various biological pathways,which mainly involved substance metabolism(including fatty acids,amino acids and so on)and energy metabolism,especially the metabolism of amino acid and fatty acid.This study would provide data support for further research on cold resistance mechanism of alfalfa.