老年克罗恩病患者肠组织中lncRNA CCAT1的表达与预后的关系

Relationship between expression of lncRNA CCAT1 in intestinal tissues and prognosis in elderly patients with Crohn's disease

目的检测老年克罗恩病(CD)患者肠组织中结肠癌相关转录因子1(CCAT1)的表达水平,并分析其与患者预后的关系。方法选择2017年3月至2019年3月在空军军医大学第一附属医院确诊的93例老年CD患者作为研究对象。根据患者的预后情况将其分为预后良好组(n=52)和预后不良组(n=41)。采用实时荧光定量PCR法检测CD患者肠道炎性组织及正常组织中CCAT1的表达水平。采用ROC曲线评估CCAT1判断CD患者预后的效能。采用多因素logistic回归法分析影响患者预后的危险因素。结果CD患者炎性组织中CCAT1的表达水平显著高于正常组织,差异有统计学意义(P<0.05)。预后不良组的炎性组织中CCAT1的表达水平显著高于预后良好组,差异有统计学意义(P<0.05)。CCAT1判断CD患者预后的ROC曲线下面积(AUC)、敏感度和特异度分别为0.932、0.902和0.923。多因素logistic回归分析结果显示,疾病行为、肛周病变、首次治疗时使用糖皮质激素及CCAT1均为影响CD患者预后的独立危险因素(P均<0.05)。结论检测CD患者炎性组织中CCAT1的表达水平有助于判断患者的预后情况。

Objective This paper aims to detect the expression level of colon cancer-associated transcription factor 1(CCAT1)in the intestinal tissue of elderly patients with Crohn's disease(CD),and to analyze its relationship with the prognosis of the patients.Methods A total of 93 elderly patients with CD who were diagnosed in the First Affiliated Hospital of Air Force Medical University from March 2017 to March 2019 were selected as the research subjects.According to the prognosis of patients,they were assigned to the good prognosis group(n=52)and the poor prognosis group(n=41).The expression levels of CCAT1 in intestinal inflammatory tissues and normal tissues of CD patients were detected by real-time fluorescence quantitative PCR.The ROC curve was used to evaluate the efficacy of CCAT1 in judging the prognosis of CD patients.Multivariate logistic regression was used to analyze the risk factors affecting the prognosis of patients.Results The expression level of CCAT1 in inflammatory tissues of CD patients is significantly higher than that in normal tissues,with a statistically significant difference(P<0.05).The expression level of CCAT1 in the inflammatory tissue of the poor prognosis group is significantly higher than that of the good prognosis group,with a statistically significant difference(P<0.05).The area under the ROC curve(AUC),sensitivity and specificity of CCAT1 in judging the prognosis of CD patients are 0.932,0.902,and 0.923,respectively.The results of multivariate logistic regression analysis show that the type of lesions,perianal lesions,the use of glucocorticoids in the first treatment,and CCAT1 are all independent risk factors for the prognosis of CD patients(P<0.05).Conclusion Detection of the expression level of CCAT1 in inflammatory tissue of CD patients is helpful to judge the prognosis of patients.