肺岩宁方联合顺铂对肺癌A549细胞生物学行为的影响

Effects of Feiyan Ning Formula combined with Cisplatin on biological behavior of lung cancer cell line A549

目的:探讨肺岩宁方及顺铂对肺癌A549细胞增殖、迁移、凋亡等细胞生物学行为的影响。方法:体外培养人肺腺癌A549细胞,运用CCK-8细胞增殖实验筛选肺岩宁方和顺铂的优化给药浓度及时间,检测药物对A549细胞增殖抑制作用;运用划痕实验检测药物对细胞迁移能力的影响;利用流式细胞术测定药物对细胞凋亡的影响;运用Hoechst染核,扫描激光共聚焦显微系统观察细胞凋亡形态学的改变。结果:与空白对照组比较,肺岩宁方组和顺铂组均能抑制A549细胞增殖活力(P<0.01),联合用药组对肺癌细胞增殖的抑制强于肺岩宁方组(P<0.01)。与空白对照组比较,各药物组干预肺癌细胞后迁移能力显著降低(P<0.05,P<0.01),联合用药较肺岩宁方更为显著(P<0.01)。联合用药组肺癌细胞凋亡率显著高于肺岩宁方组(P<0.01)。与空白对照组比较,各药物组肺癌细胞凋亡被促进,联合用药促进凋亡的效果更明显。结论:肺岩宁方具有调控A549细胞生物学行为的作用,肺岩宁方显著抑制肺癌A549细胞的增殖活力及迁移能力,促进肺癌细胞凋亡,与顺铂联合应用具有协同增效作用。

Objective:To explore the effects of Feiyan Ning Formula(FYN),Cisplatin and their combination on the lung cancer cell biological behavior of human non-small cell lung cancer(NSCLC)cell line A549(proliferation,transfer,apoptosis,etc.).Methods:Human lung adenocarcinoma A549 cells were cultured in vitro.CCK-8 method was used to detect the optimal concentration FYN,Cisplatin and the survival of lung cells treated with single drug and their combination.The inhibitory effect of the drugs on the migration of A549 cells were tested by scratch test.The effects of FYN and Cisplatin on the apoptosis rate of A549 cells were detected by flow cytometry.The effects of drugs intervention on the morphology of A549 cells were observed by Hoechst staining and laser scanning confocal microscopy.Results:Compared with control group,FYN and Cisplatin group could inhibit the growth of A549 cells(P<0.01),and the inhibition of united group was significantly stronger than that of the FYN group(P<0.01).Compared with control group,the change of scratch distance was significantly decreased after drugs intervention in A549 cells(P<0.05,P<0.01),the inhibition of united group was significantly stronger than that of the FYN group(P<0.01).The rate of apoptosis of lung cancer cells was significantly higher in the combination group than that in the FYN group(P<0.01).Compared with control group,the apoptosis of lung cancer cells was promoted by each drug group,and the effect of combined drugs on apoptosis was more obvious.Conclusion:FYN can regulate the biological behavior of A549 cells.FYN can significantly inhibit the proliferation activity and migration of A549 cells,promote the apoptosis of lung cancer cells,and has synergistic effect when combined with Cisplatin.