附子中1个新的苯基异喹啉生物碱及其心肌保护活性

A novel phenylisoquinoline alkaloid with cardioprotective effect from Aconiti Lateralis Radix Praeparata

采用大孔树脂柱色谱、硅胶柱色谱、制备薄层色谱和半制备高效液相色谱等分离技术从生附片Aconitum carmichaelii酸水提取物中分离得到1个新的9-苯基异喹啉生物碱(1)和1个已知的pavine型异喹啉生物碱(2),并运用多种现代波谱技术对化合物结构进行鉴定,通过计算ECD确定其绝对构型,鉴定为(aS)-7,8-二甲氧基-9-(2-羧基-4,5-二甲氧基苯基)-3,4-二氢异喹啉-1(2H)-酮(1)。采用阿霉素诱导的H9c2大鼠心肌细胞损伤模型评价化合物1和2的心肌细胞保护作用,两者均表现出不同程度的心肌保护活性。

Macroporous resin chromatography,silica gel column chromatography,preparative thin layer chromatography,and semi-preparative high performance liquid chromatography were performed to isolate two compounds from the acid extract of the lateral roots of Aconitum carmichaelii:a new 9-phenylisoquinoline alkaloid(1)and a known pavine alkaloid(2).Their structures were elucidated by spectroscopy.The absolute configuration of compound 1 was identified by electronic circular dichroism(ECD)and it was determined to be(aS)-7,8-dimethoxy-9-(2-carboxy-4,5-dimethoxyphenyl)-3,4-dihydroisoquinoline-1(2H)-one(1).The cardioprotective effects of 1 and 2 against doxorubicin-induced toxicity in H9 c2 cells were evaluated.Both of the isoquinoline alkaloids showed cardioprotective activity.