摘要
[Objectives]A method for the determination ofβ-sitosterol in Plumbago zeylanica L.was established and the content ofβ-sitosterol in different medicinal parts,different producing areas and different harvest periods were compared.[Methods]High performance liquid chromatography(HPLC)-evaporative light scattering detector assay was used.The chromatographic column was Kromasil C_(18) column(250 mm×4.6 mm,5μm);the mobile phase was pure methanol;the flow rate was 1.0 mL/min;the column temperature was 30℃.The detection parameters of evaporative light scattering detector were as follows:drift tube temperature was 40℃,carrier gas(N_(2))pressure was 3.5 bar.[Results]There was a good linear relationship betweenβ-sitosterol(1.080-4.860μg)and the natural logarithm of peak area(r=0.9995).The average recovery rate was 99.80%.The content ofβ-sitosterol in root and stem was 0.2074 and 0.4064 mg/g,respectively,but it was not found in leaves;the content ofβ-sitosterol in P.zeylanica L.in Guangxi was generally lower than that in Yunnan,and the content ofβ-sitosterol in P.zeylanica L.in Xishuangbanna was the highest;the content ofβ-sitosterol in the stem of P.zeylanica L.was stable at a relatively high level in different harvest periods.[Conclusions]The method is simple,accurate and reproducible,and can be used as one of the methods to control the quality of P.zeylanica L.
