烟叶中多菌灵胶体金定量试纸条的研制及应用

Colloidal gold test strip to quantify carbendazim residues in tobacco leaves:development and application

为了建立烟叶中残留的多菌灵的快速定量检测方法,利用胶体金免疫层析技术并配套胶体金读数仪,确定更适用于现场检测的多菌灵试纸条的产品形式,明确温度对胶体金试纸条定量结果的影响,用恒温孵育器控制检测温度,基于胶体金读数仪绘制标准曲线。结果表明:①研制的定量胶体金试纸条检测限为0.017 mg/kg,检测范围为0.020~2.0 mg/kg,定量范围为0.020~0.50 mg/kg,加标回收率为95.6%~128.9%,批内变异系数为6.2%~11.2%;②与高效液相色谱-串联质谱法的检测结果相比,阴性样品检测结果准确率大于95%,阳性样品的检测结果在定量范围0.020~0.50 mg/kg内线性关系良好;③对于与多菌灵结构相似的药物,试纸条与其无交叉反应,可特异性定量检测烟叶中残留的多菌灵。本研究中研制的多菌灵定量试纸条具有操作简便、检测速度快、准确性高、稳定性好等优点,适用于基层实验室的大批量样品检测及现场快速检测。

In order to establish a rapid and quantitative test method to measure carbendazim residues in tobacco leaves for field detection,carbendazim test strip based on colloidal gold immunochromatography technology and colloidal gold reader were developed and evaluated.The influence of temperature on the quantitative results by the colloidal gold test strip was clarified,and the standard curve of the colloidal gold reader was set by controlling the detection temperature with a constant temperature incubator.The results showed that:1)The detection limit of the developed quantitative colloidal gold test strip was 0.017 mg/kg with the detection range of 0.020-2.0 mg/kg and the quantitative range of 0.020-0.50 mg/kg.The standard recovery was 95.6%-128.9%and intra assay coefficient of variation was 6.2%-11.2%.2)Compared with the results tested by using high performance liquid chromatography tandem mass spectrometry,the test result accuracy of negative samples was higher than 95%,and the test results of positive samples had a good linear correlation in the range of 0.020-0.50 mg/kg.3)The test strip had no cross reaction with similarly structured drugs,therefore it could be used to detect the carbendazim residues in tobacco leaves.The developed quantitative test strip had the advantages of simple to use,fast detection speed,high accuracy and good stability,and is thus suitable for the detection of large quantities of samples in primary laboratories and field detection.