香菇多糖联合奥沙利铂对EC109细胞增殖作用的研究

Inhibitory Effect of Lentinan Combined with Oxaliplatin on EC109 Cells

目的:探究香菇多糖(LNT)联合奥沙利铂(OXA)对人食管癌细胞EC109增殖、周期、凋亡的影响。方法:采用Chou-Talalay联合用药指数法计算LNT联合OXA最佳作用浓度。将EC109细胞分为NC组(空白组)、OXA组、LNT组、LNT+OXA联合用药组,显微镜观察药物对细胞形态的影响,MTT法检测药物对细胞增殖的影响,流式细胞术检测药物对EC109细胞周期和细胞凋亡的影响。结果:LNT和OXA对EC109细胞均具有抑制增殖作用,并呈现剂量依赖性;Compusyn软件分析发现LNT(1200μg·mL^(-1))和OXA(20μg·mL^(-1))处理细胞24 h时作用效果最佳;与NC组、OXA组、LNT组相比LNT+OXA组细胞数量显著减少且体积变大,抑制增殖作用显著增强,将细胞周期阻滞在G_(2)期,细胞凋亡率显著升高。结论:香菇多糖联合奥沙利铂对EC109细胞具有明显的抑制增殖、促进凋亡的作用。

Objective:To investigate the effects of Lentinan(LNT)combined with Oxaliplatin(OXA)on the proliferation,cell cycle,and apoptosis of human esophageal cancer cell line EC109.Methods:MTT assay was used to evaluate the effects of Lentinan and oxaliplatin on the proliferation of EC109 cells.The optimal concentration of LNT combined with OXA was calculated by using Chou-Talalay combined drug index method.EC109 cells were divided into NC group(blank group),OXA group,LNT group,and LNT+OXA combined treatment group.The effect of drugs on cell morphology was observed under the microscope,and the effects of drugs on cell proliferation were detected by the MTT method.The cell cycle and apoptosis rate of EC109 were detected by flow cytometry.Results:Both LNT and OXA inhibited the proliferation of EC109 cells in a dose-dependent manner.Compusyn software analysis showed that LNT(1200μg·mL^(-1))and OXA(20μg·mL^(-1))had the best effect when treated for 24 h.Compared with the NC group,OXA group,and LNT group,the number of cells in the LNT+OXA group was significantly reduced and the volume became larger and rounder;the inhibition of proliferation was significantly enhanced;the cell cycle was blocked in the G_(2) phase;the apoptosis rate was significantly increased.Conclusion:Lentinan combined with oxaliplatin can significantly inhibit proliferation and promote apoptosis of EC109 cells.