胶质母细胞瘤组织RBM8A表达与病人预后及替莫唑胺化疗敏感性的关系

Relationship between the RBM8A expression in glioblastoma tissues and the prognosis and sensitivity of temozolomide chemotherapy of patients with glioblastoma

目的探讨胶质母细胞瘤(GBM)组织RNA结合基序8A(RBM8A)蛋白表达与病人预后及替莫唑胺(TMZ)化疗敏感性的关系。方法选取2015年6月至2020年6月手术切除的GBM组织130例及颅脑损伤减压术中切取的非肿瘤脑组织20例(对照),用免疫组化法检测组织RBM8A表达。术随访时间3~50个月,中位随访时间为12个月。结果GBM组织RBM8A高表达率[86.15%(112/130)]明显高于对照组[20%(4/20);P<0.05]。随访期间死亡98例,失访2例;多因素Cox回归分析显示,RBM8A高表达是GBM病人生存预后不良的独立危险因素(P<0.05)。生存曲线分析显示,RBM8A高表达GBM病人中位无复发生存时间和总生存时间均明显低于低表达病人(P<0.05);RBM8A低表达GBM病人中,TMZ化疗病人无复发生存时间和总生存时间均明显高于未化疗病人(P<0.05);TMZ化疗与RBM8A高表达GBM病人无复发生存时间和总生存时间无明显关系(P>0.05)。结论GBM组织RBM8A呈高表达,与病人不良生存预后有关。检测RBM8A有助于评估GBM病人对TMZ化疗敏感性。

Objective To investigate the relationship between the RNA binding motif 8A(RBM8A)expression in glioblastoma tissues and the prognosis and sensitivity of temozolomide chemotherapy of patients with glioblastoma.Methods The RBM8A expressions were detected using immunohistochemicai staining in the glioblastoma tissues obtained from 130 patients with glioblastoma who underwent micorsurgery from January 2014 to June 2020 and in non-tumor cerebral tissues obtained from 20 patients with traumatic brain injury who underwent decompression(control group).The glioblastoma patients were followed up for 3~50 months,with an medium time of 12 months.Results The high expression rate of RBM8A in glioblastoma tissues[86.15%(112/130)]was significantly higher than that in control group[20%(4/20);P<0.05].During the follow-up,98 patients died and 2 were lost to follow-up.Multivariate Cox regression analysis showed that high expression of RBM8A was an independent risk factor for poor survival prognosis of glioblastoma patients(P<0.05).Survival curve analysis showed that the median recurrence-free survival time and overall survival time of glioblastoma patients with high expression of RBM8A were significantly lower than those of patients with low expression of RBM8A(P<0.05).Temozolomide chemotherapy had no significant effect on the recurrence-free survival time and overall survival time of glioblastoma patients with high RBM8A expression(P>0.05).Conclusions The expression of RBM8A in glioblastoma tissue is high,which is related to the poor survival prognosis of glioblastoma patients.Detection of RBM8A is helpful to assessing the sensitivity of glioblastoma patients to temozolomide chemotherapy.