摘要
基于金属有机框架具有模拟酶的特性,构建实时检测胆固醇电化学传感器。采用自组装的方法制备出新型的复合金属有机框架模拟酶(Fe_(3)O_(4)@Au/MOF)纳米粒子,其兼具了Fe_(3)O_(4)磁性粒子的可回收特点以及金纳米粒子的加速电子转移等优点。并利用红外光谱、透射电子显微镜以及X射线光电子能谱对制备成功的Fe_(3)O_(4)@Au/MOF的结构和形貌进行表征。借助Fe_(3)O_(4)@Au/MOF的类过氧化物酶双重催化性能使胆固醇氧化为胆甾烯三酮和H_(2)O_(2),而H_(2)O_(2)在其进一步的催化下产生的具有氧化活性的羟基自由基可促进3,3′,5,5′-四甲基联苯胺发生氧化还原反应,进而增加了体系中电荷的移动。根据电信号的响应情况,从而实现对胆固醇的快速定性和高效定量的检测。研究结果表明,采用循环伏安法测得优化检测体系为:反应温度为50℃,Fe_(3)O_(4)@Au/MOF添加量为0.0125 g,扫描速率为0.10 V/s,缓冲溶液pH=4;当胆固醇浓度处于0.001~1.500 mmol/L时呈现良好的线性关系,线性回归方程为y=20.6401x+4.7722,R^(2)=0.9956,最低检测限为2.7μmol/L;且检测体系具有良好的回收率和抗干扰性,在食品分析和生化分析等方面具有良好的应用前景。
A real-time electrochemical sensor for detecting cholesterol was constructed due to the mimic enzyme characteristics of the metal organic framework(MOF).A new-type composite metal-organic framework(Fe_(3)O_(4)@Au/MOF)nanoparticle was prepared by self-assembly method,which had the advantages of recyclability of Fe_(3)O_(4) magnetic particles and accelerating electron transfer of Au nanoparticles.The structure and morphology of the successfully prepared Fe_(3)O_(4)@Au/MOF were characterized by infrared spectroscopy,transmission scanning electron microscopy and X-ray photoelectron spectroscopy.With the dual catalytic performance of Fe_(3)O_(4)@Au/MOF peroxidase-like enzymes,cholesterol was oxidized to cholestyrtrione and H_(2)O_(2).The oxidatively active hydroxyl radical produced by H_(2)O_(2) can promote oxidation-reduction reaction of 3,3′,5,5′-tetramethylbenzidine(TMB),which increased the movement of charges in the system.According to the response of the electrical signal,the rapid qualitative and efficient quantitative detection of cholesterol can be realized.The research results showed that the optimal detection system determined by cyclic voltammetry was that the reaction temperature of 50℃,the addition amount of Fe_(3)O_(4)@Au/MOF of 0.0125 g,scanning rate of 0.10 V/s and buffer solution pH of 4.The working curve equation was y=20.6401x+4.7722,R^(2) was 0.9956,and the minimum detection limit was 2.7μmol/L,when the cholesterol concentration located in the range of 0.001~1.500 mmol/L.The detection system had a good recovery rate and anti-interference with a good application prospects in food analysis and biochemical analysis.
作者
张悦
关桦楠
刘晓飞
刘树萍
崔琳琳
宋岩
ZHANG Yue;GUAN Hua'nan;LIU Xiaofei;LIU Shuping;CUI Linlin;SONG Yan(College of Food Engineering, Harbin University of Commerce, Harbin 150028, China)
出处
《食品科学技术学报》
EI
CAS
CSCD
北大核心
2022年第4期159-168,共10页
Journal of Food Science and Technology
基金
黑龙江省科技重大专项项目(2019ZX08B02)
国家自然科学基金资助项目(31201376)
黑龙江省自然科学基金资助项目(C2016034)
中央支持地方高校专项基金项目(YSL036)
黑龙江省博士后科研启动项目(LBH-Q19027)
黑龙江省领军人才支持计划(2020376)项目
哈尔滨商业大学“青年创新人才”支持计划项目(2019CX05)
哈尔滨商业大学研究生科研创新项目(HSD20210705)。
关键词
金属有机框架
模拟酶
电化学传感器
胆固醇
食品分析
metal organic framework
mimic enzyme
electrochemical sensor
cholesterol
food analysis