Hyper-seq:A novel,effective,and flexible marker-assisted selection and genotyping approach

Recently,a novel extremely low-cost,effective,flexible,and high-throughput DNA sequencing library preparation and genotyping approach has been developed named Hyper-seq.This new technology has been adopted by more than 15 research institutes and universities,which has significantly improved the efficiency of crop breeding.The collection and explanation of all DNA sequences froma species,known as pangenome,is the future and hotspot of genomics research.The effective and flexible identification of distinct genetic materials among individuals or populations from pangenome is crucial for exploring genetic and molecular biological processes.Single-nucleotide polymorphisms(SNPs)have become the preferred genetic component to examine humandiseases aswell as animal and plant resistance andmolecular breeding.1 Meanwhile,driven by next-generation sequencing(NGS)technology,the cost of DNA sequencing has been greatly decreased.Over the past decade,genomics-assisted breeding(GAB)has played an important role in utilizing the potential and characterization of modern genomic resources.