碳酸酐酶在红细胞分化发育过程中的功能研究

Research on the Function of Carbonic Anhydrase During Erythroid Differentiation

目前红系分化调控相关的研究主要集中在细胞因子、转录因子、lncRNA及表观遗传方面,为了对红系分化调控机制进行更加深入的解析,研究了碳酸酐酶在红系分化中的功能。碳酸酐酶可以高效催化二氧化碳的水合,但它在红细胞发育过程中的功能尚不清楚。利用脐带血来源的CD34^(+)细胞在体外进行红细胞诱导分化,在分化过程中通过慢病毒介导的基因敲降的方法能够降低碳酸酐酶1和碳酸酐酶2的表达,并使用流式细胞仪检测红细胞的生成和分化效率。研究结果表明,与对照组相比,碳酸酐酶1的表达缺陷使红细胞的晚期分化明显受阻,而碳酸酐酶2的表达缺陷则将红细胞的分化阻滞在早期阶段。研究结果表明,虽然作用窗口不同,但碳酸酐酶1和碳酸酐酶2在红系分化的过程中均发挥着重要的调控作用,这一发现对将来在体外红细胞生成具有指导意义。

The current research about the regulation of erythroid differentiation mainly focuses on cytokines,transcription factors,lncRNAs and epigenetics.To further dissect mechanisms about erythroid differentiation,the function of carbonic anhydrase during erythroid differentiation was investigated.Carbonic anhydrase can catalyze the hydration of carbon dioxide,but its function in erythroid development is unclear.Erythroid differentiation of CD34^(+)cells was induced in vitro.Lentivirus-mediated knockdown of carbonic anhydrase 1 and carbonic anhydrase 2 were performed in this study.The differentiation efficiency was detected by flow cytometry.Results showed that the knockdown of carbonic anhydrase 1 led to a significant decline in late erythroid differentiation.However,erythroid differentiation was blocked in the earlier stage after carbonic anhydrase 2 knockdown.Thus,our study demonstrated that carbonic anhydrase 1 and carbonic anhydrase 2 play important roles in different stages of erythroid differentiation and this finding will be instructive for erythroid generation in vitro in the future.