基于壳聚糖-三聚磷酸盐的鱼油纳米颗粒制备及性能表征

Preparation and Characterization of Fish Oil Nanoparticles Based on Chitosan Tripolyphosphate

以不同分子质量壳聚糖-三聚磷酸盐(CS-TPP)纳米颗粒为包封壁材制备鱼油纳米颗粒,并对鱼油包封率及纳米颗粒理化性质进行研究。先采用O/W乳液技术在CS溶液中形成鱼油液滴,再利用CS与TPP的静电作用形成负载鱼油的纳米颗粒。通过红外光谱证实鱼油在纳米颗粒中的包覆,用动态光散射仪研究纳米颗粒的粒径、PDI和电位,采用浊度法计算鱼油的包封率。结果表明,在0~1.875%的鱼油添加范围内,纳米颗粒的粒径均随着鱼油添加量的增加而增大。在考察的CS分子质量范围(15~80 ku)内,CS分子质量越大,所制备的纳米颗粒粒径越小,体系分散性越好,也越稳定。其中,在鱼油添加量为1.125%时,使用80 ku的CS制备的纳米颗粒电位达到–23.33 mV。包封率随CS分子质量的增大而增大,分子质量为80 ku的CS,在鱼油添加量为0.375%~1.125%时,包封率均超过53%。包封率又会随鱼油添加量的增大而降低,为保证包封率,鱼油的添加量控制在1.125%以下为宜。试验结果证实了CS-TPP用作鱼油包封壁材的可行性,为功能性脂质的稳定化提供了思路。

The preparation process of fish oil nanoparticles with different molecular weight chitosan tripolyphosphate(CS-TPP)was studied,and the entrapment efficiency and physicochemical properties of the nanoparticles were analyzed.In the process,fish oil droplets were formed in CS solution by O/W emulsion technology,and then CS-TPP nanoparticles loaded with fish oil were formed by the electrostatic interaction between CS and TPP.The coating of fish oil in nanoparticles was confirmed by Fourier transform infrared spectroscopy(FTIR).The average particle size,polymerization dispersion coefficient(PDI),and zeta potential of nanoparticles were investigated by Malvern dynamic light scattering(DLS),and the entrapment efficiency of fish oil was calculated by nephelometry.The results showed that the average particle size of nanoparticles increased with the increase of fish oil addition in the range of 0~1.875%.In the investigated chitosan(15~80 ku),the larger the molecular weight of chitosan,the smaller the particle size of the nanoparticles,and the better the dispersion and stability of the system.Among them,the potential of nanoparticles with the chitosan of 80 ku reached–23.33 mV at 1.125%fish oil addition.The entrapment efficiency increased with the increase of molecular weight of chitosan.The entrapment efficiency of chitosan with molecular weight of 80 ku exceeded 53%when the addition amount of fish oil was 0.375%~1.125%.The entrapment efficiency decreased with the increase of the amount of fish oil.In order to ensure the entrapment efficiency,it was appropriate to control the amount of fish oil added below 1.125%.The results confirmed the feasibility of CS-TPP to be used as wall materials for fish oil encapsulation,which provides ideas for the stabilization of functional lipids.