太子参内生菌RPB-32发酵代谢物对小鼠血液生化指标的影响

Effects of Metabolites of Endophytic Bacterium RPB-32 from Radix Pseudostellariae on Blood Biochemical Indexes in Mice

【目的】探讨太子参内生菌RPB-32发酵代谢物对小鼠血液生化指标的影响。【方法】依次用石油醚、乙酸乙酯和正丁醇萃取菌株RPB-32营养肉汤发酵液,减压脱溶制得太子参内生菌RPB-32各有机溶剂提取物。用3%乙醇(溶剂对照)配制一定浓度的各有机溶剂提取物,灌胃KM小鼠,在第7天和第14天分批次摘除眼球采血,使用全自动生化分析仪检测血液生化指标。【结果】不同有机提取物3个浓度处理组的总蛋白(TP)、白蛋白(ALB)、丙氨酸氨基转移酶(ALT)、谷草转氨酶(AST)和尿素氮(BUN)与溶剂对照组比较,差异无统计学意义(P>0.05);正丁醇提取物高剂量组碱性磷酸酶(ALP)活性低于溶剂对照组,其差异具有统计学意义(P<0.05)。【结论】太子参内生菌RPB-32发酵代谢物对机体无损害作用,这提示其可能具有预防动脉硬化、高血压和糖尿病及保护肾脏、肝脏和抑制大肠杆菌等有害菌、促进乳酸杆菌等有益菌生长等功效,但其具体机制需待进一步研究。

【Objective】To investigate the effect of fermentation metabolites of endophytic bacterium RPB-32 from Radix Pseudostellariae on blood biochemical indexes in mice.【Methods】Petroleum ether,ethyl acetate and n-butanol were successively used to extract the broth of strain RPB-32;The organic solvent extracts of endophytic bacterium RPB-32 were prepared by decompressing.A certain concentration of organic solvent extracts were prepared with 3% ethanol(solvent control),and then gavaged into KM mice.The eyeballs were removed in batches on the 7th and 14th days,and the blood biochemical indexes were detected by automatic biochemical analyzer.【Results】Of different organic extracts,there was no significant difference in total protein(TP),albumin(ALB),alanine aminotransferase(ALT),aspartate aminotransferase(AST)and urea nitrogen(BUN)between the three concentration treatment groups and the solvent control group(P>0.05);The activity of alkaline phosphatase(ALP)in the high-dose n-butanol extract group was lower than that in the solvent control group,and the difference was statistically significant(P<0.05).【Conclusion】The metabolites of endophytic bacterium RPB-32 of Radix Pseudostellariae heterophylla had no harmful effect on the body.This suggests that it may have the effects of preventing arteriosclerosis,hypertension and diabetes,protecting kidney and liver,inhibiting harmful bacteria such as bacillus coli,and promoting the growth of beneficial bacteria such as Lactobacillus,but its specific mechanism needs to be further studied.