组蛋白去乙酰化酶9在鼻咽癌中的表达及其临床意义

Expression and Clinical Significance of Histone Deacetylase(HDAC9)in Nasopharyngeal Carcinoma

【目的】探讨组蛋白去乙酰化酶9(HDAC9)在鼻咽癌中的表达及其临床意义。【方法】选取两院经病理活体组织检查确诊的41例鼻咽癌患者为观察组,另选取慢性鼻咽炎患者24例作为对照组;采用免疫组化法检测两组患者HDAC9蛋白表达水平;采用实时荧光定量PCR(qRT-PCR)检测HDAC9mRNA的相对表达量。分析并比较HDAC9mRNA高表达组与低表达组患者综合治疗后3年生存情况。【结果】观察组的HDAC9蛋白表达量明显低于对照组,且差异有统计学意义(P<0.05);观察组HDAC9mRNA的相对表达量亦明显低于对照组(P<0.05)。鼻咽癌组织标本中HDAC9mRNA高表达例数为30例,远高于低表达例数11例;HDAC9mRNA表达与性别、年龄、病理分型等无关(P>0.05),与淋巴结转移、TNM分期有关(P<0.05)。随访显示,HDAC9mRNA高表达患者的3年生存率为56.67%(17/30),低表达的3年生存率为81.82%(9/11),高表达组生存率低于低表达组,但两者相比较差异无统计学意义(P>0.05)。【结论】HDAC9在鼻咽癌患者中呈低表达,HDAC9mRNA表达与淋巴结转移、TNM分期有关。

【Objective】To explore the expression of histone deacetylase 9(HDAC9)in nasopharyngeal carcinoma and its clinical significance.【Methods】A total of 41 patients with nasopharyngeal carcinoma confirmed by pathological biopsy in Otorhinolaryngology of our hospital were selected as the observation group.In addition,24 patients with chronic nasopharyngitis were selected as the control group.The expression of HDAC9 protein was detected by immunohistochemistry;the relative expression of HDAC9 mRNA was detected by real-time fluorescent quantitative PCR(qRT-PCR).The three-year survival of nasopharyngeal carcinoma patients in the HDAC9 mRNA high expression group and the HDAC9 mRNA low expression group were compared and analyzed.【Results】Immunohistochemistry showed that the expression of HDAC9 protein in the observation group was significantly lower than that in the control group(P<0.05);qRT-PCR showed that the relative expression of HDAC9 mRNA in the observation group was significantly lower than that in the control group(P<0.05).The number of cases with high expression of HDAC9 mRNA(n=30)was much higher than that of cases with low expression(n=11)in nasopharyngeal carcinoma tissues(P<0.05).The expression of HDAC9 mRNA was not related to gender,age and pathological type(P>0.05),while its expression was related to lymph node metastasis and TNM stage(P<0.05).The 3-year survival rate of patients with high expression of HDAC9 mRNA was 56.67%(17/30)and the 3-year survival rate with low expression was 81.82%(9/11).The survival rate of HDAC9 mRNA high expression group was lower than that of low expression group(P>0.05).【Conclusion】The expression of HDAC9 is low in nasopharyngeal carcinoma patients,and the expression of HDAC9 mRNA is related to lymph node metastasis and TNM stage.