急性淋巴细胞白血病患者骨髓单个核细胞中miR-17、ATG12水平及其临床意义

Levels and Significance of miR-17 and ATG12 in Bone Marrow Mononuclear Cells of Leukemia Patients

【目的】探讨成人急性淋巴细胞白血病(ALL)患者骨髓单个核细胞(BMMNC)中微小RNA-17(miR-17)、自噬基因-12(ATG12)的表达及其临床意义。【方法】收集本院收治的83例成人ALL患者骨髓标本作为观察组,根据病情将其分为初诊组(30例)、缓解组(32例)、难治复发组(21例),选取同期在本院诊治的、与观察组年龄、性别相匹配的30例骨髓正常非造血系统疾病患者的骨髓标本作为对照组。检测各组患者BMMNC中miR-17、ATG12的相对表达量,分析miR-17与ATG12相对表达量的关系。【结果】观察组miR-17、ATG12相对表达量均高于对照组(P<0.05);初诊组和难治复发组miR-17、ATG12相对表达量明显高于对照组,缓解组明显低于初诊组及难治复发组(P<0.05);缓解组miR-17、ATG12相对表达量与对照组比较,差异均无统计学意义(P>0.05),难治复发组miR-17、ATG12相对表达量与初诊组比较,差异均无统计学意义(P>0.05);WBC≥50×10^(9)/L的初诊患者miR-17相对表达量明显高于WBC<50×10^(9)/L的患者(P<0.05),ATG12相对表达量明显低于WBC<50×10^(9)/L的患者(P<0.05)。B-ALL患者miR-17、ATG12相对表达量均明显低于T-ALL患者(P<0.05);miR-17、ATG12相对表达量与患者性别、年龄、染色体是否异常、泼尼松诱导是否敏感无关(P>0.05);Pearson相关性分析结果显示,miR-17与ATG12相对表达量呈正相关(r=0.414,P<0.05)。【结论】成人ALL患者BMMNC中miR-17和ATG12均呈高表达,且呈正相关,ATG12的自噬活性增强,miR-17和ATG12的表达变化可能与ALL的发生、发展和复发相关,但具体的作用机制有待进一步研究。

【Objective】To investigate the expression and significance of microRNA-17(miR-17)and autophagy gene-12(ATG12)in bone marrow mono-nuclear cells(BMMNC)of adult acute lymphoblastic leukemia(ALL).【Methods】A total of 83 adult bone marrow specimens of ALL patients admitted to our hospital were collected as the observation group.According to their disease conditions,they were divided into the newly diagnosed group of 30 cases,the remission group of 32 cases,and the refractory group of 21 cases.A total of 30 bone marrow specimens from patients with normal bone marrow and non-hematopoietic system diseases who were treated in our hospital during the same period,which matched the age and sex of the observation group,were selected as the control group.The relative expressions of miR-17 and ATG12 in BMMNC were detected and the relationship of relative expressions of miR-17 and ATG12 were analyzed.【Results】The relative expressions of miR-17 and ATG12 in the observation group were higher than those in the control group(P<0.05).The relative expressions of miR-17 and ATG12 in the newly diagnosed group and the refractory relapse group were significantly higher than those in the control group(P<0.05),while the relative expressions of miR-17 and ATG12 in the remission group were significantly lower than those in the newly diagnosed group and the refractory relapse group(P<0.05).The relative expressions levels of miR-17 and ATG12 in the remission group and the control group showed no statistically significant difference(P>0.05),while the relative expressions levels of miR-17 and ATG12 in the refractory and relapsed group showed no statistically significant difference from the newly diagnosed group(P>0.05).The relative expression level of miR-17 in the newly diagnosed patients with WBC≥50×10^(9)/L was significantly higher than that in the patients with WBC<50×10^(9)/L(P<0.05),while the relative expression level of ATG12 was significantly lower than that in the patients with WBC<50×10^(9)/L(P<0.05).The relative expression level of miR-17 and ATG12 in the patients with B-ALL was significantly higher than that in the patients with T-ALL(P<0.05).The relative expression levels of miR-17 and ATG12 were independent of gender,age,chromosome abnormality and prednisone induction sensitivity(P>0.05).Pearson correlation analysis showed that the relative expression levels of miR-17 and ATG12 were positively correlated(r=0.414,P<0.05)【Conclusion】Both miR-17 and ATG12 are highly expressed and they are positively correlated in BMMNC of adult ALL patients.The autophagy activity of ATG12 was enhanced,suggesting that changes in the expression of miR-17 and ATG12 may be related to the occurrence,development and recurrence of ALL,whose specific mechanism of action remains to be further studied.