海洋放线菌Streptomyces sp.AHMU XC 2026次级代谢产物及其抗植物病原真菌活性研究

Secondary Metabolites of Marine-derived Streptomyces sp.AHMU XC 2026 and Their Anti-fungal Evaluation Against Plant Pathogenic Fungi

利用色谱法对一株海洋沉积物来源的放线菌Streptomyces sp. AHMU XC 2026次级代谢产物进行分离纯化,结合NMR波谱分析和文献比对鉴定10个单体化合物,分别为cyclo-(trans-4-OH-D-Pro-D-Phe)(1),cyclo-(D-Pro-D-Phe)(2),cyclo-(L-Ala-L-Phe)(3),cyclo-(L-leucine-L-proline)(4),cyclo-(L-Leu-trans-4-hydroxy-L-Pro)(5),cyclo-(D-Pro-D-Ile)(6),trans-2-methylcinnamic acid(7),indole-3-carboxaldehyde(8),2-pyrrolecarboxylic acid(9),2-hydroxy phenyl acetic acid(10)。采用滤纸片扩散法筛选抗植物病原真菌活性,结果表明化合物10在100μg/disc下对水稻稻瘟真菌Pyricularia oryzae HNM 1003具有微弱的抑制活性,抑制圈直径为8 mm。

An investigation for the secondary metabolites from a marine sediment-derived Streptomyces sp. AHMU XC 2026was performed, leading to an attainment of ten known compounds by NMR spectroscopic analysis and comparisons of literature data, including cyclo-(trans-4-OH-D-Pro-D-Phe)(1), cyclo-(D-Pro-D-Phe)(2), cyclo-(L-Ala-L-Phe)(3), cyclo-(L-leucine-L-proline)(4), cyclo-(L-Leu-trans-4-hydroxy-L-Pro)(5), cyclo-(D-Pro-D-Ile)(6), trans-2-methylcinnamic acid(7), indole-3-carboxaldehyde(8), 2-pyrrolecarboxylic acid(9), 2-hydroxy phenyl acetic acid(10). All the isolated compounds were evaluated on antifungal activity against a panel of four plant pathogenic fungi by an agar diffusion method, which indicated that the compound 10 showed a weak inhibitory activity for Pyricularia oryzae HNM 1003 with an inhibition zone diameter of 8 mm under 100 mg/disc.