合理推动病毒核酸检测技术的临床应用

Practically promote the clinical application of virus nucleic acid detection technology

核酸体外扩增技术给分子生物学学科带来了革命性的变更。经过近40年的相关技术的开发和应用,核酸体外扩增技术业已成为临床病毒实验室中的主导手段。非引物依赖的宏基因扩增技术、下一代核苷酸序列测定技术等病毒核酸检测技术在临床上逐渐得到更广泛的应用。为了核酸检测技术能够有效精准用于临床,一些检测前注意事项需要关注;需要开发简单、快速、准确和安全的床边/即时(point of care)检测技术;更要注意到结果的正确解读。病毒核酸检测技术的临床合理应用必将极大推动临床病毒学的发展。

In vitro nucleic acid amplification technology has brought revolutionary changes to molecular biology.After nearly 40 years of development and application,nucleic acid amplification technology has become the leading methodology in the clinical virology laboratory.Primer-independent macrogenome amplification,next-generation sequencing and other viral nucleic acid detection technologies have been widely used in the laboratory.In order to effectively and accurately use nucleic acid detection technologies in the clinic,attention must be paid to development and implementation of simple,rapid,accurate and safe bedside/point of care technology,and to correctly interpret test result.The practical application of virus nucleic acid detection technologies will greatly advance the evolution of clinical virology.