一株日化产品污染菌及其特性分析

A bacterium from contaminated daily products and its characteristic analysis

日化工厂发生无规律大规模产品变质系微生物污染引起,其污染菌可以引起防腐剂含量下降,具有一定的特殊性。为了帮助企业查找日化产品微生物污染源,有必要扩充日化产品污染菌种资源信息,建立起日化产品微生物防控的科学基础。利用菌落总数计数分析了解其微生物污染情况;使用16S rRNA基因测序的方法鉴定优势菌种,高通量测序方法分析污染样品的群落构成。使用液相色谱法分析样本中防腐剂的含量并通过最低抑菌浓度试验和防腐功效挑战试验,测试了污染菌的耐药性及防腐体系对该菌种的抵抗力。该批微生物污染的样品中菌种总数已超过104 CFU/g。不同样品的污染菌群落构成有细微差别,但优势菌均为类产碱假单胞菌,其占比在53.59%~99.9%之间。该菌种可以引起配方中的异噻唑啉酮类防腐剂的含量下降,对该类防腐剂具有耐药性,污染菌的最低抑菌浓度为40 mg/kg~60 mg/kg,而标准菌的最低抑菌浓度值仅为5 mg/kg。添加污染菌混合标准菌作为防腐挑战菌种时,对现有产品的防腐体系有冲击作用,挑战失败的样本中防腐剂含量均降低至不能检出,残留菌种为铜绿假单胞菌。类产碱假单胞菌是本批产品污染的主要污染来源,污染菌对现用的卡松类防腐剂有耐药性,并可以引起体系中的异噻唑啉酮类防腐剂含量下降。现有的防腐体系不足以对产品形成充分有效的保护,需进一步筛选新的防腐体系。该菌种具有一定的非典型性,对于丰富现有的菌种资源信息和该类微生物污染的治理有积极的意义。

The irregular large-scale product deterioration occurred in daily chemical plants was caused by microbial contamination,which could decrease of the preservative content and had certain particularity.In order to help the enterprises to find microbial polluted source in daily chemical products,it is necessary to expand the information of microbial polluted resources in daily chemical products for establishing a scientific basis of microbial prevention and control.The microbial contamination was analyzed by colony count analysis,the dominant bacteria were identified by 16S rRNA gene sequencing,and the community composition of contaminated samples was analyzed by high throughput sequencing.The contents of preservatives in the samples were analyzed by high performance liquid chromatography(HPLC),and the drug resistance of the contaminated bacteria and the resistance of the antiseptic system to the bacteria were tested by minimum inhibitory concentration(MIC)test and antiseptic efficacy challenge test.The total number of bacteria in the samples contaminated by microorganisms had exceeded 104 CFU/g.There were slight differences in the community composition of contaminated bacteria in different samples,but the dominant bacteria were Pseudomonas pseudoaligenes,accounting for 53.59%to 99.9%.The total numbers of the bacteria in the polluted products were more than 104 CFU/g.There were slight differences in the community composition of contaminated bacteria among different samples,but the dominant bacteria were Pseudomonas pseudoaligenes,accounting for 53.59%to 99.9%.These contaminated bacteria could reduce the content of isothiazolinone preservatives in the formula,and had drug resistance to these preservatives.The minimum inhibitory concentration of contaminated bacteria was 40 mg/kg~60 mg/kg,while that of standard bacteria was only 5 mg/kg.When the standard bacteria mixed with contaminated bacteria were added as antiseptic challenge bacteria,it had an impact on the antiseptic system of existing products.The content of preservatives in the failed samples decreased to such an extent that they could not be detected,and the residual strain was Pseudomonas aeruginosa.Pseudomonas pseudoaligenes was the main source of contamination in this batch of products.These contaminated bacteria were resistant to current carbazone preservatives and could cause the decrease of the content of isothiazolinone preservatives in the system.The existing preservation system was not enough to protect products effectively,so it was necessary to further screen a new one.This strain was atypical to a certain extent,which had the positive significance for enriching the information of existing bacteria resources and the treatment of this kind of microbial pollution.